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P2356 Sigma

Protein Synthesis: Methods and Protocols

  •  ISBN-10 0-89603-397-X

  •  ISBN-13 978-0-89603-397-9

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publication info   R. Martin, ed., Humana Press, 1998, 456 pp., hard cover
mfr. no.   Humana

Description

General description

This book presents a collection of molecular biological methods specific to protein synthesis. Chapters open with a discussion of basic background information and strategy which is then complemented by comprehensive methodological details. The book is divided into seven significant areas that cover all of the research techniques required by both experienced researchers and newcomers to the field of protein synthesis, and will prove to be an invaluable reference source on the benchtop of many protein laboratories.

Table of Contents

Analyses of Ribosome Distribution During In Vitro Translation,
Allele-Specific Priming in the Mapping of rRNA,
Permeabilized Mammalian Cells as a System for Protein Synthesis,
The Measurement of Processivity Errors in Protein Synthesis,
A Biosynthetic Approach for the Incorporation of Unnatural Amino Acids into Proteins,
The Analysis of Translational Activity Using a Reporter Gene Constructed from Repeats of an Antibody-Binding Domain from Protein A,
In Vitro Engineering Using Acyl-Derivatized tRNAs,
In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Antocodons,
Analysis of rRNA Function Using Specialized Ribosomes, Determination of the Peptide Elongation Rate In Vivo,
The Measurement of Missense Errors During In Vivo Protein Synthesis,
Heelprinting Analysis of In Vivo Ribosome Pause Sites.
Analysis of eIF-2a Kinases in Yeast. Continuous-Flow Cell-Free Translation,
Transcription-Translation, and Replication-Translation Systems.
Analysis of Translational Activity of Extracts Derived from Oocytes and Eggs of Xenopus laevis.
A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors,
Measurement of Ribosomal Accuracy and Proofreading in E. coli Burst Systems,
Measurement of Rate of Protein Synthesis In Vitro Preparation of Escherichia coli Burst Systems,
In Vivo Mutational Analysis of Ribosomal RNA in Saccharomyces cerevisiae,
Genetic Selection of rRNA Mutations,
Toeprinting Assays: Mapping by Blocks to Reverse Transcriptase Primer Extension,
Mapping of Pseudouridine Residues in RNA to Nucleotide Resolution, Chemical and Enzymatic Probing of Antibiotic-Ribosome Complexes.
Protein Engineering with Nonstandard Amino Acids. Internal Ribosome Entry Sites Tests with Circular mRNAs,
Gel Retardation and UV-Crosslinking Assays to Detect Specific RNA-Protein Interactions in the 5′ or 3′ UTRs of Translationally Regulated mRNAs,
Translational Control by Repressor Proteins Binding to the 5′ UTR of mRNAs,
Identification and Analysis of Frameshift Sites,
Synthesis and Site-Specific Binding of Thioated tRNAs to Probe Ribosome-tRNA Interactions,
Index.

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