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  • P247 - Monoclonal Anti-phospho-CaM Kinase IIα (pThr286) antibody produced in mouse

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P247 Sigma

Monoclonal Anti-phospho-CaM Kinase IIα (pThr286) antibody produced in mouse

clone 22B1, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, CAM-CB,
species reactivity   mouse, rat
application(s)   indirect immunofluorescence: 1:100 using rat hippocampus (cold methanol fixation)
  western blot: 1:2,000
clone   22B1, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~50 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Camk2a(12322)
rat ... Camk2a(25400)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

phosphorylated (at Thr286) synthetic phosphopeptide corresponding to amino acids of rat and mouse CaM kinase IIα subunit that contains threonine 286.

General description

Intracellular Calcium (Ca2+) acts as major secondary messenger that mediates signalling induced by hormones and neurotransmitters. Cellular responses to calcium are modulated by a family of Ca2+/Calmodulin dependent protein kinases. The members of this family include CaMK I, CAMK II and CAMK IV. These kinases mediate rapid, highly specific and reversible post-translational modification involving ATP. CaMK II is abundantly expressed in mammalian brain, with highest expression in neurons of hippocampus and cerebral cortex, and plays critical role in synaptic transmission, memory, learning and behaviour. CaMK II family has four members, CaMK IIα, CaMK IIβ, CaMK IIγ and CaMK IIδ. The CaMK IIα?and β?isoforms are predominantly expressed in the brain, localized mainly in the cytosol and postsynaptic densities (PSDs), whereas the CaMK IIγ and δ isoforms are expressed in all tissues. CaMK II is autophosphorylated at α-Thr286 residue in response to changes in the concentration of intracellular calcium. This may prolong physiological responses to temporary changes in Ca2+ concentrations. CaMK II regulates synaptic signalling by phosphorylating glutamate receptors in the neurons. A distinct CaMK II has also been implicated in the process of smooth muscle contraction by phosphorylation of myosin light chain kinase.
This product reacts with the recombinant and native rat phosphorylated CaMK II α subunit only (50 kDa). It does not cross-react with the non-phosphorylated α subunit or with the 60 kDa β subunit in either phosphorylation state.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Specificity

The antibody reacts specifically with both the recombinant and native rat phosphorylated CaM (Ca2+/calmodulin) dependent kinase IIα subunit. It does not cross-react with the non-phosphorylated α subunit or with the β subunit (60 kDa) in either phosphorylation state. By immunofluorescence on rat hippocampal cells, the antibody labels cell somas and neuropil areas, while nuclei are only lightly stained.

Application

Anti-phospho-CaM Kinase IIα (pThr286) antibody may be used in indirect immunofluorescence at a working dilution of 1:100 in rat hippocampal cells fixed with cold methanol. Detection by immunoblotting is possible at a working dilution of 1:1000.

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Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  nwg
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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