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  • P2482 - Monoclonal Anti-Protein Kinase Bα antibody produced in mouse

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P2482 Sigma

Monoclonal Anti-Protein Kinase Bα antibody produced in mouse

clone PKB-175, ascites fluid

Synonym: Anti-Akt1, Anti-PKBα

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Properties

Related Categories AKT Antibodies, AKT1 Antibodies, Alphabetical Index, Antibodies, Antibodies for Apoptosis,
species reactivity   bovine, rat, mouse, human, chicken
application(s)   indirect ELISA: suitable
  microarray: suitable
  western blot: 1:5,000 using human MCF-7 cell extract
clone   PKB-175, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 56 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... AKT1(207)
mouse ... Akt1(11651)
rat ... Akt1(24185)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

Synthetic peptide corresponding to amino acids 461-477 of human PKBα/Akt1, conjugated to KLH. The sequence is identical in mouse, rat, and bovine PKBα/Akt1, highly conserved in PKBβ/Akt2, and diverges in PKBγ.

General description

Protein Kinase B (also known as Akt, or RAC-PK) is a protein that belongs to serine/threonine kinaseswhich regulate many functions like-cell proliferation and differentiation, controlling cell cycle and apoptosis. It also has a pivotal role in protein synthesis and glycogen synthesis. Anti-protein kinase B α antibody can be used for studying the expression and function of PKB/Akt isoforms in different cells and tissues. It can also be used in microarray. Mouse anti-protein kinase B α antibody reacts specifically with an epitope present in the C-terminal amino acids 461-477 of human PKB α/Akt1 (56kD). This product is also reactive for PKB α/Akt1 of rat, bovine, mouse and chicken.

Application

Anti-protein kinase B α antibody can be used in indirect ELISA and western blotting (diluted 1:5000) using human MCF-7 cell extract.

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Safety Information

WGK Germany  2
Protocols & Articles

Articles

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A valuable measure of the knock-down potency of any RNAi experiment is the reduction in protein level. Displayed below are the knock-down rates of  selected esiRNAs using quantitative western blot an...
Keywords: Cell proliferation, Gene expression, PAGE, Reductions, Transfection, Western blot

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