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P248 Sigma

Monoclonal Anti-PARP antibody produced in mouse

clone C-2-10, ascites fluid

Synonym: Monoclonal Anti-Poly[ADP-ribose] Polymerase

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to PARP,
species reactivity   hamster, primate, mouse, rat
does not react with   avian
application(s)   immunocytochemistry: 1:1,000
  indirect ELISA: suitable
  western blot: 1:1,000
clone   C-2-10, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 116 kDa (PARP)
  antigen mol wt 85 kDa (prICE/caspase-3 cleavage product)
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Parp1(11545), Parp2(11546)
rat ... Parp1(25591), Parp2(290027)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

calf thymus PARP. The epitope is found between amino acids 216-375 in the DNA-binding domain of PARP.

Physical form

Solution in mouse ascites fluid containing 0.02% sodium azide.

Specificity

The antibody reacts with PARP (116 kDa) and the 85 kDa apoptosis-induced cleavage product of prICE (proteinase resembling interleukin 1β-converting enzyme) and caspase-3 (CPP32 cysteine protease).

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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References

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