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  • P2584 - Monoclonal Anti-Protein Kinase Cβ2 antibody produced in mouse

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P2584 Sigma

Monoclonal Anti-Protein Kinase Cβ2 antibody produced in mouse

clone PK-B26, ascites fluid

Synonym: Anti-PKC β2

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, PR-PR,
species reactivity   rat
application(s)   microarray: suitable
  western blot: 1:8,000 using rat brain cytosol preparation
clone   PK-B26, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 80 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Prkcb(25023)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminal variable (V5) region (amino acids 660-673) of PKC β2.

General description

Protein Kinase C (PKC, 76-93 kD) is a family of Ser/Thr specific protein kinases that perform key functions in numerous signalling pathways, in biological systems, through their various isoforms. The conventional PKC isoforms (cPKC) are PKC-α, β1, β2 and γ; activated by phosphatidylserine, calcium or phorbol esters. Proteolysis of PKC in vivo is thought to be mediated by calpains I and II. Calpains cleave PKC in the V3 hinge region to produce two distinct fragments, one comprising the N-terminal regulatory domain (30 kD) and the other fragment containing the C-terminal kinase domain (50 kD) that is catalytically active. Multiple functions such as, cellular and vascular regulations, angiogenesis, cell growth, apoptosis, changes in basement membrane thickness, extracellular matrix organisation, MAPK signalling, are attributed to PKC isoforms. These varied functions implicate PKC isoforms in cardiac hypertrophies and diabetic nephropathy and cardiovascular complications.
Anti-Protein Kinase C β2 antibody specifically recognizes an epitope located within the amino acid residues 660-673 at the C-terminal variable (V5) region of PKCβ2 (80 kDa).

Specificity

The antibody shows no cross-reactivity with PKC peptides corresponding to C-terminal sequences from PKC β1 (658-671) and PKC γ (684-697) conjugated to BSA.

Application

Anti-Protein Kinase C β2 antibody may be used for immunoblotting at a working dilution of 1:8000 using rat brain cytosolic extract. It is suitable for protein microarray.

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