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P2611 Sigma

Pectinase from Aspergillus aculeatus

aqueous solution, ≥3,800 units/mL

Synonym: Pectinex® Ultra SPL

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Description

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

250 mL in glass bottle

Legal Information

A product of Novozyme Corp.

Pectinex is a registered trademark of Novozymes Corp.

Application

Pectinase is an enzyme from Aspergillus aculeatus that is used in plant protoplast preparation to digest cell wall prior to organelle isolation. It has been used to conduct partial saccharification of sugars. Pectinases are used to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications. Pectinase, product P2611, has been used to treat leaf discs from fifth wild-type and sitiens leaves.

Biochem/physiol Actions

Pectinase is an active pectolytic enzyme preparation produced by a selected strain of Aspergillus aculeatus that contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinase hydrolyzes pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Selective Extraction and Low Level Quantification of the Mycotoxin Patulin from Organic Apple Puree

Patulin (Figure 1) is a mycotoxin produced by several genera of mold including blue Penicillium species. The toxin was discovered and characterized in the 1940’s during routine general research condu...
K. G. Espenschied, Olga Shimelis, Michael Ye, Jennifer Claus
Reporter US Volume 33.2
Keywords: Antibiotics, Centrifugation, Chromatography, Filtration, Gastrointestinal, Genetic, High performance liquid chromatography, Sample preparations, Solid phase extractions

Related Content

Enzyme Explorer Key Resources: Lysing Enzymes

Cell Lysis Enzymes – How to tear down the Wall Enzymes provide a non-mechanical method for cell lysis and protoplast preparation. It may seem like a simple process to throw in your enzyme, stick your...
Keywords: Amplification, Biochemistry, Cell culture, Cell disruption, Culture media, Enzymology, Metabolites, Molecular biology, PAGE, Purification

Peer-Reviewed Papers
15

References

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