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P2645 Sigma

Protein Kinase A Catalytic Subunit from bovine heart

≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)

Synonym: PKA

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Description

Packaging

Package size based on phosphorylating units

Preparation Note

Prepared from protein kinase A (P 5511)

Unit Definition

One unit will transfer 1.0 picomole of phosphate from γ-32P-ATP to hydrolysed and partially dephosphorylated Casein per min at pH 6.5 at 30 °C.

Physical form

Lyophilized powder containing ≤ 1% protein with sucrose and phosphate buffer salts as stabilizer

Application

Protein Kinase A (PKA) Catalytic Subunit from bovine heart has been used-
• to study PKA-mediated inhibition of IRK1 (inwardly rectifying K+) channels
• in in Vitro PKA pPhosphorylation assay
• in Vitro affinity binding assays
• to study effects of PKA on inspiratory drive currents in functionally active motorneurons

Biochem/physiol Actions

Protein Kinase A catalyzes the transfer of terminal phosphate from ATP to threonine or serine residues present on various proteins. This protein is inactive in the absence of cAMP, where the catalytic and regulatory subunits are bound together. The regulatory subunit, in the presence of cAMP, binds to cAMP and releases the catalytic subunit.

General description

Protein Kinase A enzyme is composed of two subunits- catalytic and regulatory. The catalytic subunit exists as a monomer in the presence of cAMP and has a molecular weight of 40,862 Da.

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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