• USA Home
  • P2645 - Protein Kinase A Catalytic Subunit from bovine heart

P2645 Sigma

Protein Kinase A Catalytic Subunit from bovine heart

≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)

Synonym: PKA




Package size based on phosphorylating units

Preparation Note

Prepared from protein kinase A (P 5511)

Unit Definition

One unit will transfer 1.0 picomole of phosphate from γ-32P-ATP to hydrolysed and partially dephosphorylated Casein per min at pH 6.5 at 30 °C.

Physical form

Lyophilized powder containing ≤ 1% protein with sucrose and phosphate buffer salts as stabilizer


Protein Kinase A (PKA) Catalytic Subunit from bovine heart has been used-
• to study PKA-mediated inhibition of IRK1 (inwardly rectifying K+) channels
• in in Vitro PKA pPhosphorylation assay
• in Vitro affinity binding assays
• to study effects of PKA on inspiratory drive currents in functionally active motorneurons

Biochem/physiol Actions

Protein Kinase A catalyzes the transfer of terminal phosphate from ATP to threonine or serine residues present on various proteins. This protein is inactive in the absence of cAMP, where the catalytic and regulatory subunits are bound together. The regulatory subunit, in the presence of cAMP, binds to cAMP and releases the catalytic subunit.

General description

Protein Kinase A enzyme is composed of two subunits- catalytic and regulatory. The catalytic subunit exists as a monomer in the presence of cAMP and has a molecular weight of 40,862 Da.

Price and Availability

Your Key to Success

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers


Related Products


Product #


Add to Cart

M6760 Myricetin, ≥96.0%, crystalline
P0393 Protein Kinase Inhibitor from porcine heart, 1-3 inhibitor U/μg protein, lyophilized powder
C1172 ML-9, ≥99% (TLC), powder
G1274 HA-1004 hydrochloride, ≥98% (HPLC), powder
M9656 H-8 dihydrochloride, >98% (HPLC)
C2932 Chelerythrine chloride, ≥95% (TLC), powder
I7016 H-7 dihydrochloride, ≥98% (HPLC)
B1427 H-89 dihydrochloride hydrate, ≥98% (HPLC), powder
P0300 Protein Kinase Inhibitor from rabbit, ≥85% (HPLC)
I6891 H-7
I2764 ML-7, powder

related product

Product #


Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?