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P2714 Sigma

Protease Inhibitor Cocktail powder

for general use, lyophilized powder

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin

Application

Optimized and tested for general use.

Biochem/physiol Actions

This mixture contains individual components, including AEBSF at 2 mM, Aprotinin at 0.3 μM, Bestatin at 116 μM, E-64 at 14 μM, Leupeptin at 1 μM and EDTA at 1 mM. Each component has specific inhibitory properties.

AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.

Caution

The lyophilized powder is stable for 4 years when stored at -20°C.

Packaging

1 bottle in serum bottle

Preparation Note

This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.

Quantity

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Specificity

Inhibits serine, cysteine, and metallo-proteases.

Price and Availability


Pharma QC

Ultrafree Centrifugal Filter Units

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

How much of Product P2714, Protease Inhibitor Cocktail, needs to be used in cell lysates?
One bottle is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract. This and other useful information can be found at: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/5/p2714dat.pdf
How should I dissolve and store Product P2714, Protease Inhibitor Cocktail?
You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use.  Aliquots can be stored at -20 C for 1 month.  This and other useful information can be found on the product data sheet:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/5/p2714dat.pdf
How much of Product P2714, Protease Inhibitor Cocktail, should I use with serum?  
Dilute the contents of the vial in 10 mL of water, yielding a 10X initial concentration . Then dilute the stock 1:10 in the serum (1 part of inhibitor solution to 10 parts of serum). This would be a 1:100 dilution (containing the concentrations of each inhibitor as listed on the data sheet) in the serum. This is true for most cell lysates, serum, and culture media.
Can Product P2714, Protease Inhibitor Cocktail, be used with ELISA?
Yes, because it is water based.
Can Product P2714, Protease Inhibitor Cocktail,be used with Nickel columns?
No because it contains EDTA.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the difference between Protease Inhibitors S8820 and P2714?
S8820 is the tablet version of P2714.  
Show more questions
Protocols & Articles

Articles

Protease Inhibitor Cocktails References

The Sigma-Aldrich portfolio of protease inhibitor cocktails shows an excellent track record in the peer-reviewed scientific literature, as witnessed by the many publications that cite our protease in...
Keywords: AGE, Adhesion, Aerobic, Affinity chromatography, Alzheimer Disease, Amplification, Angiogenesis, Anti-inflammatory agents, Antibiotics, Antimicrobials, Antitumor agents, Antivirals, Apoptosis, Atomic absorption spectroscopy, Autophagy, Biochemistry, Biological processes, Buffers, Cancer, Cardiovascular, Catalysis, Cell biology, Cell disruption, Cell proliferation, Cell signaling, Cellular processes, Central Nervous System, Chromatin immunoprecipitation, Chromatography, Clinical, Cloning, Cytotoxins, DNA replication, Degradations, Detergents, Diabetes, Diagnostic, Diseases, Dopamine agents, Drug discovery, Eliminations, Environmental, Enzyme-linked immunosorbent assay, Gas chromatography, Gastrointestinal, Gene expression, Genetic, Genetics, Gluconeogenesis, Glycosylations, Growth factors, Hormones, Hydration reaction, Hydroxylations, Immobilization, Immunology, Immunoprecipitation, Inflammation, Ligands, Lipid Metabolism, Lipid peroxidation, Mass spectrometry, Medicinal chemistry, Metabolism, Metabolites, Methylations, Microarray Analysis, Microbiology, Microscopy, Molecular biology, Morphogenesis, Neurology, Neuroscience, Neurotransmission, Neurotransmitters, Normal-phase chromatography, Nutrition, Obesity, Oncology, PAGE, Parkinson Disease, Peroxidations, Pharmaceutical, Phosphorylations, Photosynthesis, Physiological Processes, Polymerase chain reaction, Polymorphisms, Proteomics, Purification, Recombination, Reductions, Reproduction, Respiratory, Reversible addition-fragmentation chain transfer polymerizations, Sample preparations, Scanning electron microscopy, Schizophrenia, Separation, Sequencing, Sonication, Transcription, Transduction, Veterinary, Vitamins, Western blot, transformation

Peer-Reviewed Papers
15

References

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