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P2850 Sigma

Phosphatase Inhibitor Cocktail 1

DMSO solution

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

Tested on cell extracts from animal tissues such as bovine liver, human placenta, and rabbit muscle, as well as on extracts from A431 and Jurkat cell lines.

General description

A mixture of inhibitors that will inhibit the L-isozymes of alkaline phosphatase as well as serine/threonine protein phosphatases such as PP1 and PP2A. Contains microcystinLR, cantharidin, and (−)-p-bromotetramisole. This product has been replaced with P0044.

Quantity

Recommended usage is a 100-fold dilution.

Price and Availability


Pharma QC

Ultrafree Centrifugal Filter Units

All labs need water
Safety & Documentation

Safety Information

RIDADR 
UN 1993 / PGIII
WGK Germany 
1
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How stable is Product P2850, Phosphatase Inhibitor Cocktail 1?  Can I freeze it?
This reagent, as supplied and stored at 2-8°C, will be stable for four years from the date of manufacturing. Freezing is not recommended, because it may cause precipitation of the inhibitors. To view this information on our website, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/p2850dat.Par.0001.File.tmp/p2850dat.pdf
At what concentration should I use Product P2850, Phosphatase Inhibitor Cocktail 1?
In most circumstannces, the product is used at a 100-fold dilution.  This will inhibit the phosphatases in 5 mg of protein per mL of lysate. To view this information on P2850 data sheet, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/p2850dat.Par.0001.File.tmp/p2850dat.pdf
Product P2850, Phosphatase Inhibitor Cocktail 1, is solid when it arrived. Is it suitable to use?
Since this solution is supplied in DMSO, it may solidify (melting point of DMSO is 16-19 deg C).  Simply allow it to melt before use.  This will not affect the product.
What inhibitors are present in Product P2850, Phosphatase Inhibitor Cocktail 1, and in what concentrations?
Cantharidin (Product Code C7632) inhibits protein phosphatase 2A (PP-2A).  Bromotetramisole (Product Code B8132) inhibits alkaline phosphatases. Microcystin LR (Product Code M2912) inhibits protein phosphatases 1 and 2A (PP-1 and PP-2A). The concentrations of these inhibitors are considered proprietary. To view this information on the P2850 data sheet, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/p2850dat.Par.0001.File.tmp/p2850dat.pdf
Is Product P2850, Phosphatase Inhibitor Cocktail 1, suitable for use on yeast? 
As far as we know, the phosphatases in mammalian cells also appear in yeast cells. Thus both cocktails, P5726 (Phosphatase cocktail II and P2850 (Phosphatase Inhibitor cocktail I), may be needed with yeast extracts.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Phosphatase Inhibitor Cocktail

Phosphorylation is a reversible protein post-translational modification. Protein kinases catalyze the transfer of the negatively charged γ-phosphate group from ATP to a hydroxyl side chain of serine,...
Ian Gleiser, Pnina Yaish, Efrat Barnea-Gedalyahu, Dorit Zharhary
Biowire Volume 10 Article 1
Keywords: Apoptosis, Biochemistry, Cell signaling, Cellular processes, Clinical, Clinical Chemistry, Degradations, Diseases, Enzymology, Medicinal chemistry, Metabolism, Methods, Molecular biology, Phase transitions, Phosphorylations, Post translational modifications, Purification, Transduction

Peer-Reviewed Papers
15

References

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