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P2859 Sigma

Monoclonal Anti-p300/CBP antibody produced in mouse

clone NM11, purified from hybridoma cell culture

Synonym: Anti-CREB Binding Protein

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Chromatin Remodeling, Antibodies for Gene Regulation,
species reactivity   human, mink, monkey, rat, mouse
application(s)   immunocytochemistry: suitable
  immunoprecipitation1,2: suitable
  microarray: suitable
  western blot1,2: 10-20 μg/mL using human 293 embryonal kidney cells
clone   NM11, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 300 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... KAT2B(8850)
mouse ... Kat2b(18519)
rat ... Pcaf(301164)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

native human p300.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Reacts specifically with both p300 and CBP but not with the related p270 molecule. The epitope recognized by the antibody resides within the 21 amino acid stretch spanning amino acids 2071-2091 near the CBP C-terminus. CBP and p300 differ by three noncontiguous residues within this 21 amino acid region, a difference that does not detectably affect the reactivity of the antibody.

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