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P2860 Sigma

Monoclonal Anti-PSF antibody produced in mouse

clone B92, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Gene Regulation, Antibodies to Nuclear Proteins, Epigenetics,
biological source   mouse
antibody form   purified immunoglobulin
clone   B92, monoclonal
form   buffered aqueous solution
species reactivity   human, mouse
concentration   ~2 mg/mL
application(s)   immunocytochemistry: suitable
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  microarray: suitable
  radioimmunoassay: suitable using cells
  western blot: 2-4 μg/mL using whole extract of cultured HeLa cells
isotype   IgG1
conjugate   unconjugated
storage temp.   −20°C

Description

Immunogen

lysate of mouse bone marrow-derived stromal cell line.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)

Monoclonal Anti-PSF antibody is useful in affinity chromatography, immunoblotting, RIA and immunoprecipitation. It can also be used in immunocytochemistry and immunohistochemistry.

General description

PSF (polypyrimidine tract-binding protein-associated splicing factor) is an ubiquitous nuclear matrix protein which plays a pivotal role in mammalian spliceosome. The PTB/PSF complex formation is also necessary for pre-mRNA splicing. Monoclonal anti-PSF antibody can be used as a primary antibody in immunofluorescence staining. This antibody is also useful in affinity chromatography, immunoblotting and immunocytochemistry. Monoclonal anti-PSF antibody reacts specifically with human and mouse PSF.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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