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P2893 Sigma

JumpStart Taq ReadyMix

Complete optimized reagent for hot-start PCR at 2X concentration

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Properties

Related Categories Core Bioreagents, Hot Start PCR, Life Science Reagents for PCR, Molecular Biology, PCR Enzymes,
form   liquid
feature   hotstart
concentration   2.5 units/reaction (50 μL reaction volume)
color   colorless
Featured Industry   Agriculture
shipped in   wet ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

• For PCR amplifications that require reduced non-specific amplification
• For multiplex PCR
• For reduction of primer dimers

Features and Benefits

• Reduce set-up time, eliminate contamination concerns, and avoid long reactivation steps with JumpStart Readymixes
• JumpStart Taq antibody allows for room temperature set up of reactions making it ideal for high throughput applications
• Prevents amplification of non-specific products and primer dimers, increasing target yields

Other Notes

JumpStart Taq ReadyMix is a prepared solution combining the performance benefits of hot start PCR with the convenience of a ReadyMix. The mix includes JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. JumpStart Taq Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Unlike other hot-start methods (i.e. chemical inactivation), JumpStart Taq polymerase does not require a pre-incubation step prior to cycling because polymerase activity is fully restored during the first denaturation cycle of the PCR reaction. The hot start mechanism allows for room temperature set up, making it ideal for high throughput applications. To prepare a 50 μL reaction, simply add 25 μL of ReadyMix to template, primers and water for a final reaction volume of 50 μL.

View more detailed information on JumpStart Taq ReadyMix products at www.sigma-aldrich.com/hotstart.

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

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Protocols

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Hot Start Taq Polymerase Protocol to Reduce Non-Specific Amplification

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Peer-Reviewed Papers
15

References

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