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P2979 Sigma

Anti-PAK3 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous glycerol solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, P-PA,
species reactivity   rat, mouse
application(s)   immunoprecipitation: 4 μg using 0.5 mg of mouse brain cytosol
  western blot (chemiluminescent): 2 μg/mL using mouse 3T3 fibroblasts extract or mouse brain extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous glycerol solution
mol wt   antigen mol wt 65 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   mouse ... Pak3(18481)
rat ... Pak3(29433)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 1-13 of the N-terminal of mouse p21 activated kinase 3 (PAK3).

General description

p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK.
Anti-PAK3 specifically reacts with mouse and rat PAK3 (65 kDa). It reacts weakly with human PAK3.

Physical form

Solution in 0.07 M Tris-glycine, pH 7.4, containing 30% glycerol and 0.035% sodium azide.

Application

Anti-PAK3 may be used at a concentration of 4 μg to immunoprecipitate PAK3 from 0.5-1 mg mouse NIH3T3 total cell lysate. It may be used at a concentration of 1-2 μg/mL for detection in total mouse NIH3T3 lysate by immunoblotting.

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