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P3039 Sigma

Peroxidase Anti-Peroxidase Soluble Complex antibody produced in mouse

affinity isolated antibody, buffered aqueous solution

Synonym: PAP

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Properties

Related Categories Alphabetical Index, Antibodies, PAP, PD-PG, Primary Antibodies,
biological source   mouse
antibody form   affinity isolated antibody
form   buffered aqueous solution
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
shipped in   dry ice
storage temp.   −20°C

Description

Physical form

Solution in 8 mM sodium acetate, 15 mM ammonium acetate buffered saline, pH 7.2, containing 0.5% bovine serum albumin and 0.01% thimerosal.

Application

Sigma PAP is assayed by immunoelectrophoresis, Ouchterlony double diffusion, and immunohistochemical staining techniques to ensure purity and functional reactivity. It is recommended that the optimal working dilution be determined in the specific assay system.

Biochem/physiol Actions

The Peroxidase Anti-Peroxidase (PAP) soluble complex is prepared by a modification of the method of Sternberger using specific polyclonal or monoclonal antibodies to horseradish peroxidase and highly purified enzyme. Since the enzyme is not covalently coupled to the antibody, the soluble complex retains its enzyme and antibody reactivity with distinct advantages over enzyme-conjugated antibodies.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

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Peer-Reviewed Papers
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References

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