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P3110 Sigma

Anti-PDK1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories 3-Phosphoinositide-Dependent Protein Kinase (PDK), Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
species reactivity   human
application(s)   microarray: suitable
  western blot: 1:1,000 using recombinant human PDK1
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 60 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PDPK1(5170)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminus of human PDK1 (amino acids 538-556).

General description

The 3-phosphoinositide-dependent protein kinase-1 (PDK1, PKB kinase), is a master regulator of AGC kinases. It mediates cellular effects by activating major pathways involving PKB/Akt , S6K, RSK, SGK and the PKC isoforms. PDK1 is ubiquitously present in cells and is constitutively active. PDK1 is a critical activator of PKB/Akt as it mediates phosphorylation at Thr308 in the catalytic domain, thus plays a significant role on oncogenic signalling by PI3K, cell growth, survival, proliferation and migration. By regulating AGC kinases PDK1 is actively involved in the regulation of protein translation and metabolism, and serum and gluco-corticoid induced signalling.
Anti-PDK1 recognizes human PDK1 (60 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-PDK-1 may be used for detection by immunoblotting at a working dilution of 1:1000. The antibody is suitable for protein microarray.

Price and Availability


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