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P3203 Sigma

Anti-Protein Kinase Cβ2 antibody produced in rabbit

0.5 mg/mL, affinity isolated antibody

Synonym: Anti-PKC β2

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, PR-PR,
species reactivity   rat
application(s)   dot blot: 1:1,000
  microarray: suitable
  western blot: 1:8,000 using rat brain extract
clone   polyclonal
concentration   0.5 mg/mL
antibody form   affinity isolated antibody
mol wt   antigen mol wt 80 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Prkcb(25023)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminal region of PKC β2 (660-673).

General description

Protein Kinase C (PKC, 76-93 kD) is a family of Ser/Thr specific protein kinases that perform key functions in numerous signalling pathways, in biological systems, through their various isoforms. The conventional PKC isoforms (cPKC) are PKC-α, β1, β2 and γ; activated by phosphatidylserine, calcium or phorbol esters. Proteolysis of PKC in vivo is thought to be mediated by calpains I and II. Calpains cleave PKC in the V3 hinge region to produce two distinct fragments, one comprising the N-terminal regulatory domain (30 kD) and the other fragment containing the C-terminal kinase domain (50 kD) that is catalytically active. Multiple functions such as, cellular and vascular regulations, angiogenesis, cell growth, apoptosis, changes in basement membrane thickness, extracellular matrix organisation, MAPK signalling, are attributed to PKC isoforms. These varied functions implicate PKC isoforms in cardiac hypertrophies and diabetic nephropathy and cardiovascular complications.
Anti-Protein Kinase C β2 antibody specifically recognises PKC β2 (80 kDa).

Physical form

Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Specificity

In immunoblotting, the product reacts with PKC β2 (80 kDa) using rat brain extract.

Preparation Note

Purified by affinity chromatography using immobilized PKC β2 peptide.

Application

Anti-Protein Kinase C β2 antibody may be used for detection by immunoblotting at a working dilution of 1:8000 using rat brain extract. It may be used in dat blot at a working dilution of 1:1000. It is suitable for protein microarray.

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