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P3226 Sigma

Protein L-Peroxidase from Peptostreptococcus magnus

recombinant, expressed in E. coli, buffered aqueous glycerol solution



Related Categories Antibodies, Protein A, G and L Detection Reagents, Secondary Antibodies More...
recombinant   expressed in E. coli
form   buffered aqueous glycerol solution
human IgG binding affinity   2-3 × 109 M-1
application(s)   direct ELISA: 1:3,000
  dot blot: suitable
  western blot: suitable
solubility   PBS, pH 7.2: soluble (May be diluted in buffer)
conjugate   peroxidase conjugate
shipped in   wet ice
storage temp.   −20°C


Biochem/physiol Actions

Protein L binds immunoglobulins from a variety of sources primarily through κ light chain interactions without interfering with the antigen binding site of Igs. This means that Protein L binds to a wider range of Ig classes and subclasses from a variety of species than any other commercially available Ig binding protein. It contains four Ig binding domains (B-domains). Protein L does not bind to bovine, sheep or goat immunoglobulins. Protein L-Peroxidase Conjugate may be used for the detection of IgG, IgM, IgA, IgE and IgD containing κ light chains. It may be used to detect Fab and scFv fragments containing κ light chains.

Preparation Note

Prepared with recombinant protein L conjugated to horseradish peroxidase using glutaraldehyde coupling chemistry.


Binds Igs with κ light chain only. Does not bind λ light chain.

Physical form

Solution in 0.01 mM phosphate bufferered saline, pH 7.2, containing 50% glycerol.

Price and Availability

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Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


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