|Related Categories||Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins,|
|antibody form||ascites fluid|
|contains||15 mM sodium azide|
|application(s)||indirect ELISA: 1:1,600|
|western blot: 1:2,000 using a fresh human platelet preparation|
|shipped in||dry ice|
Monoclonal anti-phosphotyrosine antibody produced in mouse is used in immunohistochemisty, flow cytometry, immunoprecipitation, immunoblotting, ELISA and RIA for localization of phosphorylated tyrosine containing proteins. It can also be used for immunoaffinity isolation.1
Phosphorylation, attachment of a phosphate group to protein, alters protein functionality by activation or deactivation of the protein, in which protein kinase plays a crucial role. Tyrosine residue phosphorylation plays a key role in cell growth and differentiation. The monoclonal anti-phosphotyrosine antibody is useful in immunoblotting for identification of phosphotyrosine containing protein from cultured human epidermoid carcinoma cell line A-431 and human platelets preparation. This product can be used in immunofluorescent labeling of tyrosine residue at focal adhesion and cellular junctions of cultured MDCK cells. This product is also useful in studies of signal transduction and growth factor receptors. This antibody has shown specificity for binding to proteins that contain phosphorylated tyrosine residue either in free amino acid form or in conjugation with KLH or BSA but will not react with non-phosphorylated tyrosine or other phosphorylated proteins and amino acids. It will also not react with phosphorylated molecules like ATP or AMP. Monoclonal anti-phosphotyrosine reacts specifically with mouse and human.
As determined by ELISA and competitive ELISA, the antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP.
Urokinase Plasminogen Activator present in tissue extracts.
phosphotyrosine conjugated to BSA
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
A Drosophila Homolog of Bovine smg p25a GDP Dissociation Inhibitor Undergoes a Shift in Isoelectric Point in the Developmental Mutant quartet Zahner, J., and Cheney, C. Mol. Cell. Biol. 13, 217-227, (1993)
Transduced PDZ1 domain of PSD-95 decreases Src phosphorylation and increases nNOS (Ser847) phosphorylation contributing to neuroprotection after cerebral ischemia. Wang, W.W., et al. Brain Res. 1328, 162-70, (2010)
Immunohistochemistry of phosphotyrosine residues: identification of distinct intracellular patterns in epithelial and steroidogenic tissues. Arad-Dann, H., et al. J. Histochem. Cytochem. 41, 513-519, (1993)
The RhoA-dependent assembly of focal adhesions in Swiss 3T3 cells is associated with increased tyrosine phosphorylation and the recruitment of both pp125FAK and protein kinase C-delta to focal adhesions. Barry, S.T., and Critchley, D.R. J. Cell Sci. 107, 2033-2045, (1994)
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