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P3363 Sigma

Protein Sequencing and Identification Using Tandem Mass Spectrometry

  •  ISBN-10 0-471-32249-0

  •  ISBN-13 978-0-471-32249-8

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Related Categories Books and Software, Labware, Proteomics Books, Spectroscopy Books
publication info   M. Kinter and N.E. Sherman, Wiley-Interscience, 2001, 301 pp., hard cover
mfr. no.   Wiley

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Readers will find in this book complete experimental detail to apply mass spectrometry techniques in their laboratoriess and learn about the proper selection and operation of instruments. Coverage includes peptide fragmentation and interpretation of product ion spectra, preparation of protein digests for sequencing experiments, mass spectrometric analysis using capillary liquid chromatography, characterization of modified peptides using tandem mass spectrometry and techniques for protein identification by database searches.

Table of Contents

An Introduction to Protein Sequencing Using Tandem Mass Spectrometry.
The Primary Structure of Proteins and a Historical Overview of Protein Sequencing.
Fundamental Mass Spectrometry.
Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra.
Basic Polyacrylamide Gel Electrophoresis.
The Preparation of Protein Digests for Mass Spectrometric Sequencing Experiments.
Mass Spectrometric Analysis of Tryptic Digests.
Protein Identification by Database Searching.
Sequence Analysis of Novel Proteins.
The Characterization of Post-Translationally Modified Proteins Using Tandem Mass Spectrometry.
Index.

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