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P3430 Sigma

Monoclonal Anti-Phosphoserine antibody produced in mouse

clone PSR-45, ascites fluid

Synonym: Monoclonal Anti-Phosphoserine, Phospho Ser, Phospho serine, Phospho−Ser, Phospho−serine

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins,
biological source   mouse
antibody form   ascites fluid
clone   PSR-45, monoclonal
contains   15 mM sodium azide
application(s)   indirect ELISA: 1:4,000
  western blot: 1:500-1:1,000
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH).

Specificity

By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site.

Application

Anti-Phosphoserine antibody may be used for indirect ELISA at a working dilution of 1:4000. For immunoblotting using rat brain cortex extracts, a working dilution of 1:500-1:1100 may be used. The antibody was used for immunoblotting to detect proteins phosphorylated at serine in stem extracts of Arabidopsis thalliana at a working dilution of 1:250.

General description

Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediate numerous signalling pathways in both prokaryotic and Eukaryotic cells. Cellular proteins with phosphorylated serine increase many fold by the activation of serine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr.
Monoclonal Anti-Phosphoserine may be used for the identification of proteins containing phosphorylated serine both as the free amino acid or when conjugated to carriers such as BSA or KLH. It does not react with non-phosphorylated serine, phosphorylated tyrosine or threonine, AMP or ATP.

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Antibody Basics

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Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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06693 Timestrip Plus -20 °C
P3717 Phosphoserine-BSA, 2 mg/mL, buffered aqueous solution

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