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P3775 Sigma

Anti-Protein A antibody produced in rabbit

fractionated antiserum, lyophilized powder

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Properties

Related Categories Alphabetical Index, Antibodies, Miscellaneous, PR-PR, Primary Antibodies More...
biological source   rabbit
antibody form   fractionated antiserum
clone   polyclonal
form   lyophilized powder
packaging   vial of 2 mL lyophilized antiserum
application(s)   indirect ELISA: 1:100,000
conjugate   unconjugated
storage temp.   2-8°C

Description

Immunogen

Protein A from Staphylococcus aureus

Reconstitution

Reconstitute with 2 ml deionized water.

Physical form

Lyophilized from 0.01M phosphate buffered saline, pH 7.2

Application

Protein A may be used as in immunoblotting with a minimum dilution of 1:50,000.

General description

The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. To ensure specificity the fractionated antiserum is adsorbed using solid phase techniques, if necessary. Rabbit Anti-Protein A is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, to which no preservatives have been added. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion versus anti-rabbit IgG results in a single arc of precipitation and versus anti-rabbit whole serum results in multiple arcs of precipitation.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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References

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