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P3813 Sigma

Phosphate buffered saline

powder, pH 7.4, for preparing 1 L solutions

Synonym: PBS

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Description

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.

Application

• Phosphate buffered saline has been used in the washing solutions during immunofluorescence procedure.
• It has been used for the suspension of bacterial cells.
• It has been used during the fixation and immunostaining procedures.

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Is Product P3813, Phosphate buffered saline, a 1 X PBS solution?
Product P3813 when prepared as directed yields a 1 X PBS solution. The answer may also be found by clicking the link:   http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/P3813
Does Product P3813, Phosphate buffered saline, contain calcium or magnesium ions?
There is no calcium nor magnesium added to the powder.  The answer can also be found by clicking:  http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/P3813
How is Product P3813, Phosphate buffered saline, supplied?
As a package of 10 foil pouches.  The answer can be also be found by clicking:  http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/P3813
What is the shelf life of Product P3813, Phosphate buffered saline?
The quality for unopened foil pouches stored at room temperature is guaranteed for one year from the time of receipt. The product is expected to be stable for at least 5 years.  The answer can also be found by clicking:  http://www.sigmaaldrich.com/Technical_Service/Technical_Service_Home/OnLine_Documents.html
How long can solutions of Product P3813, Phosphate buffered saline, be kept once prepared?
Sigma Aldrich has not evaluated the solution stability, but sterile solutions of PBS stored at 2-8 deg. C can be expected to be stable for several months.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
How much is in each pouch of Product P3813, Phosphate buffered saline?
Each pouch of phosphate buffered saline contains 8 g sodium chloride (e.g. Product No. S9625), 0.2 g potassium phosphate, monobasic (e.g. Product No. P5379), 1.15 g sodium phosphate, dibasic (e.g. Product No. S0876), 0.2 g potassium chloride (e.g. Product No. P4504), giving a total 9.55 g per packet.  This is the standard PBS formulation.
Does Product P3813, Phosphate buffered saline, contain calcium, magnesium or sodium bicarbonate?
The formulation for this phosphate buffered saline product does not contain calcium, magnesium, or sodium bicarbonate.  Sigma does not assay the product for the presence of these compounds (as impurities). None of these compounds are used in the method of preparation.
Is Product P3813, Phosphate buffered saline, sterile?
Phosphate buffered saline product number P3813 is not necessarily sterile.
Is Product P3813, Phosphate buffered saline, 1X or 10X?
Each pouch of phosphate buffered saline product number P3813 can be used to prepare 1 Liter of a 1X PBS.
Is Product P3813, Phosphate buffered saline, tested for RNase or DNase impurities?
Phosphate buffered saline product number P3813 is not tested for RNase or DNase impurities.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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