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  • P3862 - Anti-phospho-PKB (pThr308) antibody produced in rabbit

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P3862 Sigma

Anti-phospho-PKB (pThr308) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-phospho-Akt

Properties

Related Categories AKT Antibodies, AKT1 Antibodies, Alphabetical Index, Antibodies, Antibodies for Cell Biology,
species reactivity   mouse, rat
application(s)   microarray: suitable
  western blot: 1:1,000 using a whole extract of PDGF-treated mouse fibroblast NIH3T3 cells and a whole extract of H2O2-treated rat fibroblast Rat-1 cells
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 56 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... AKT1(207)
mouse ... Akt1(11651)
rat ... Akt1(24185)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic phosphopeptide corresponding to the pThr308 region of human PKBα (amino acids 301-315).

General description

Protein kinase B (PKB, also known as Akt) is a serine/threonine protein kinase and a key regulator of signal transduction processes. Important processes such as cell cycle, proliferation and apoptosis are regulated by PKB in response to stimuli such as growth factors, insulin and cytokines. Three isoforms of PKB have been identified, α, β and γ. PKB α is reportedly overexpressed in breast cancer cell lines, whereas PKB β is overexpressed in ovarian and pancreatic cancers. On cell stimulation by growth factors or insulin, PKB α translocates to the cell membrane, where it undergoes full activation by phosphorylation at Thr308 and Ser 473, by PDK1 and PDK2. PKB α plays a crucial role in several downstream pathways involved in glycogen synthesis, protein translation and cell proliferation. PKB is also a potent inhibitor of apoptotic cell death by suppressing BAD-induced death
Anti-phospho PKB (pThr308) recognises PKB phosphorylated at threonine 308 (56 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-phospho PKB (pThr308) may be used for detection and localization of phospho-PKB by immunoblotting. A minimum dilution of 1:1000 may be used for detection by immunoblotting in PDGF-treated mouse NIH3T3 cells and peroxide-treated Rat-1 fibroblast cell line.

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