P3967 Sigma


2 mg/mL, buffered aqueous solution



Related Categories Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Controls: pSer, pThr, pTyr, Molecular Biology,
form   buffered aqueous solution
concentration   2 mg/mL
application(s)   capture ELISA: 5 μg/mL using Platelet extract
  western blot: 5 μg/mL using Platelet extract
shipped in   dry ice
storage temp.   −20°C


Analysis Note

O-Phospho-L-tyrosine conjugated to bovine serum albumin


Phosphotyrosine antibody inhibitor that specifically blocks the reactivity of anti-phosphotyrosine antibody. It does not block the activity of anti-phosphothreonine or anti-phosphoserine-specific antibodies.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Phosphotyrosine-BSA may be used for specific inhibition of phosphotyrosine in platelet extracts, in immunoblotting and ELISA applications, at a working concentration of 5 μg/mL.

General description

The activity of proteins depends on phosphorylation of specific amino acid residues. In applications involving identification of activated cellular proteins, it is important to confirm specificity of antibodies used against a particular phosphorylated amino acid. For such applications, inhibitors of phosphorylated antibodies on specific amino acid residues are suitable. Phosphotyrosine antibody inhibitor may be used for the specific inhibition of the reactivity of anti-phosphotyrosine antibodies but not that of anti-phosphothreonine- or anti-phosphoserine-specific antibodies.

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Safety Information

NONH for all modes of transport
WGK Germany 


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Antibody Basics

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