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P3967 Sigma

Phosphotyrosine-BSA

2 mg/mL, buffered aqueous solution

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Properties

Related Categories Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Controls: pSer, pThr, pTyr, Molecular Biology,
form   buffered aqueous solution
concentration   2 mg/mL
application(s)   capture ELISA: 5 μg/mL using Platelet extract
  western blot: 5 μg/mL using Platelet extract
shipped in   dry ice
storage temp.   −20°C

Description

Analysis Note

O-Phospho-L-tyrosine conjugated to bovine serum albumin

Specificity

Phosphotyrosine antibody inhibitor that specifically blocks the reactivity of anti-phosphotyrosine antibody. It does not block the activity of anti-phosphothreonine or anti-phosphoserine-specific antibodies.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Phosphotyrosine-BSA may be used for specific inhibition of phosphotyrosine in platelet extracts, in immunoblotting and ELISA applications, at a working concentration of 5 μg/mL.

General description

The activity of proteins depends on phosphorylation of specific amino acid residues. In applications involving identification of activated cellular proteins, it is important to confirm specificity of antibodies used against a particular phosphorylated amino acid. For such applications, inhibitors of phosphorylated antibodies on specific amino acid residues are suitable. Phosphotyrosine antibody inhibitor may be used for the specific inhibition of the reactivity of anti-phosphotyrosine antibodies but not that of anti-phosphothreonine- or anti-phosphoserine-specific antibodies.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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References

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