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P4020 Sigma

Protamine sulfate salt from salmon

Grade X, amorphous powder

Synonym: Salmine from salmon, Salmine

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Properties

Related Categories Cell Lysis and Protein Extraction Reagents, Molecular Biology, Native Protein Sample Preparation, Protein Sample Clarification, Proteomics More...
type   Grade X
form   amorphous powder
impurities   histone, free (Millon test)
color   white to off-white
solubility   6 M HCl: soluble25 mg/mL

Description

Application

May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Biochem/physiol Actions

Protamine sulfate is a small cationic protein that binds and precipitates DNA. Neutralizes the anticoagulant activity of heparin. Inhibits lipoprotein lipase.

Packaging

1, 5, 10, 25, 100 g in poly bottle

Price and Availability


Ultrafree Centrifugal Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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