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  • P4124 - Anti-PM/Scl-100 antibody produced in rabbit

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P4124 Sigma

Anti-PM/Scl-100 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Polymyositis-scleroderma Overlap Syndrome Associated Autoantigen, Anti-Polymyositis/scleroderma Autoantigen 2, Anti-Polymyositis/scleroderma Autoantigen, 100 kDa, Anti-Autoantigen PM/Scl 2, Anti-EXOSC10, Anti-Exsosome Component 10, Anti-P100, Anti-PMSCL Autoantigen, 100-KD, Anti-PMSCL2

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics, Antibodies for Gene Regulation,
species reactivity   rat, human, mouse
application(s)   indirect immunofluorescence: 1-2 μg/mL using rat NRK and mouse NIH3T3 cells
  western blot (chemiluminescent): 1-2 μg/mL using nuclear exracts to HeLa human epithelioid carcinoma cells
clone   polyclonal
concentration   ~1.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~100 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EXOSC10(5394)
mouse ... Exosc10(50912)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acid residues 231-245 of human PM/Scl-100 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in both rat and mouse PM/Scl-100.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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