• USA Home
  • P4333 - Penicillin-Streptomycin

EMAIL THIS PAGE TO A FRIEND
P4333 Sigma

Penicillin-Streptomycin

Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, sterile-filtered, BioReagent, suitable for cell culture

Synonym: Pen-Strep

Purchase

Properties

Related Categories ADME/Tox Products, Antibacterial, Antibiotic Solutions, Antibiotics, Antibiotics A to Z,
InChI Key   KMOOCZWLFBSQCW-XFOLRDBHSA-N
sterility   sterile-filtered
product line   BioReagent
form   liquid
concentration   100 ×
impurities   endotoxin, tested
suitability   suitable for cell culture
Mode of action   cell wall synthesis | interferes
  protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
shipped in   dry ice
storage temp.   −20°C

Description

Components

This product is formulated to contain 10,000 units penicillin and 10 mg streptomycin/mL solubilized in a proprietary citrate buffer.

Application

Penicillin-Streptomycin is used to supplement cell culture media to control bacterial contamination. It is recommended for use in cell culture applications at 10 mL/L.
Penicillin streptomycin solution has been used as a supplement in different types of media, including RGC (retinal ganglion cells) culture, α-MEM (minimum essential medium) growth medium, Dulbecco′s modified Eagle′s medium (DMEM)/nutrient mixture F-12 Ham, DMEM and DMEM/F-12 + GlutaMax.

General description

Penicillin Streptomycin solution, also known as Pen Strep, is the most commonly used antibiotic solution for the culture of mammalian cells. Penicillin Streptomycin solution is used to maintain sterile conditions during cell culture, and they show few adverse effects on the cultured cells.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability


Discover the Milli-Q® IQ 7000 ultrapure water system
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Lentiviral Transduction Protocol

Prepare mammalian cells so that they are growing exponentially and are no more than 70-80% confluent before transduction.
Keywords: Cell culture, Gene expression, Molecular biology, Transduction

Related Content

Antibiotic Selection Guide

1Application Key: CC = Callus Culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant selection; SE = somatic embryogenesis; SSC = stem selection culture;...
Keywords: Antibiotics, Cell culture

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

recommended

Product #

Description

Add to Cart

CLS3401 Corning® Transwell® polycarbonate membrane cell culture inserts, 12 mm Transwell with 0.4 μm pore polycarbonate membrane insert, TC-treated, w/ lid, sterile, 48/cs
CLS3516 Corning® Costar® TC-Treated Multiple Well Plates, size 6 wells, clear, polystyrene, flat bottom, case of 50 (individually wrapped), sterile, lid
CLS2592 Corning® 1 x 8 Stripwell 96 well plates, high binding surface, clear
CLS430641 Corning® cell culture flasks, surface area 75 cm2, canted neck, cap (vented)

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?