• USA Home
  • P4377 - Phosphatase, Alkaline from Escherichia coli

EMAIL THIS PAGE TO A FRIEND
P4377 Sigma

Phosphatase, Alkaline from Escherichia coli

ammonium sulfate suspension, ~10 units/mg protein (modified Warburg-Christian)

Synonym: Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

Purchase

Description

Preparation Note

Salt fractionated

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.

Physical form

Suspension in 2.5 M (NH4)2SO4 solution

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4377 has been used for cell digestion during measurement of photoreactivating enzyme

Biochem/physiol Actions

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

WGK Germany 
3
Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

inhibitor

Product #

Description

Add to Cart

D6518 Diethylenetriaminepentaacetic acid, ≥99% (titration)
P9375 1,10-Phenanthroline monohydrate, reagent grade
E4378 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid, ≥97.0%
ED2SS Ethylenediaminetetraacetic acid disodium salt dihydrate, reagent grade, 98.5-101.5% (titration)

substrate

Product #

Description

Add to Cart

N2770 SIGMAFAST p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL
N1891 SIGMAFAST p-Nitrophenyl phosphate Tablets, tablet, To prepare 5 mL
M3168 4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System, liquid

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?