• USA Home
  • P4390 - Polynucleotide Kinase from T4-infected Escherichia coli

P4390 Sigma

Polynucleotide Kinase from T4-infected Escherichia coli

10 units/μL, buffered aqueous glycerol solution



Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
grade   for molecular biology
form   buffered aqueous glycerol solution
mol wt   mol wt 33 kDa
concentration   10 units/μL
foreign activity   Endonuclease and exonuclease, none detected
shipped in   wet ice
storage temp.   −20°C



T4 Polynucleotide Kinase is supplied in a solution of 50% glycerol (v/v), 20 mM Tris-HCl (pH 7.5), 25 mM KCl, 2mM DTT, 0.1 mM EDTA, and 0.1 μM ATP.

Analysis Note

Activity is determined in a reaction mixture containing 40 mM Tris-HCl (pH 7.5), with 10 mM MgCl2, 5 mM dithiothreitol, 0.5 mM 5’-OH polynucleotide ends, and mM [γ-32P]-ATP.


Suitable for:
• Sequencing or nucleic acid tagging (DNA and RNA) by 5′-end labeling
• 5′ phosphorylation of oligonucleotides
• Removal of 3′-phosphate groups from phosphorylpolynucleotides

Unit Definition

One unit catalyzes the transfer of one nanomole of 32P to the 5′-end of micrococcal nuclease-treated DNA in 30 min. at 37 °C. Transfer is detected as incorporation into acid-insoluble material.


Polynucleotide kinase catalyses a "forward reaction" transfer of the γ-phosphate of ATP to the 5′ hydroxyl terminus of single- and double-stranded nucleic acids (DNA and RNA) and 3′-nucleoside monophosphates. In exchange reactions containing ADP, the enzyme will catalyze the exchange of 5′-terminal phosphate groups and ATP. The 3′-phosphatase activity enables the enzyme to remove 3′-phosphoryl groups from phosphorylpolynucleotides.
1. Forward reaction: Transfer of the labeled γ-phosphate from [γ-32P]-ATP to the free 5′-hydroxyl group of the substrate.
5′-HO-DNA + [γ-32P]-ATP → 5′-32PO-DNA + ADP.
Substrates that do not have a free 5′-hydroxyl require prior dephosphorylation by alkaline phosphatase.
2. Exchange reaction: First, the terminal 5′-phosphate is transferred from the substrate to ADP present in the reaction mixture. Then, the labeled γ-phosphate from [γ-32P]-ATP is transferred to the free hydroxyl group of the substrate.
5′-PO-DNA + ADP → 5′-HO-DNA + ATP
5′-HO-DNA + [γ-32P]-ATP → 5′-32PO-DNA + ADP

Price and Availability

Your Key to Success

Biomedical Applications
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

P6911 Protease from Streptomyces griseus, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)
LIG2 QuickLink DNA Ligation Kit, joins blunt-end and sticky-end DNA fragments
LIG1 DNA Ligation Kit
D9380 DNA Polymerase I from Escherichia coli lysogenic for NM 964, buffered aqueous glycerol solution
R0884 T7 RNA Polymerase, recombinant, expressed in E. coli, buffered aqueous solution
D8276 DNA Polymerase I, Klenow Fragment from Escherichia coli, buffered aqueous glycerol solution
D2886 DNA Ligase from T4-infected Escherichia coli, buffered aqueous glycerol solution
P4978 Phosphatase, Alkaline from calf intestine, buffered aqueous glycerol solution
T4427 Terminal Transferase from calf thymus, buffered aqueous glycerol solution
E1131 Exonuclease III from Escherichia coli BE25 /psGR3, buffered aqueous glycerol solution
G5663 Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution
D7291 Deoxyribonuclease I RNase-free solution from bovine pancreas
T7428 Topoisomerase I from wheat germ, buffered aqueous glycerol solution
R6513 Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
R4642 Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?