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P4406 Sigma

Papain Agarose from papaya latex

lyophilized powder, 90-150 units/mL packed gel

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
form   lyophilized powder
mol wt   mol wt 21 kDa
extent of labeling   3,000-5,000 units per g agarose (at pH7.0 at 30 °C.)
matrix   beaded agarose
storage temp.   −20°C

Description

Application

Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P4406 is agarose-linked papain and is provided as a lyophilized powder stabilized with lactose. Product P4406 has been used to digest purified anti-JMJD6 hamster monoclonal antibody into Fab fragments.

Biochem/physiol Actions

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

Packaging

25, 50, 100 units in glass bottle

Unit Definition

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 6.2 at 25°C, unless otherwise indicated below.

Physical form

Lyophilized powder, stabilized with lactose

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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substrate

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