• USA Home
  • P4417 - Phosphate buffered saline

P4417 Sigma

Phosphate buffered saline


Synonym: PBS



Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.


Phosphate Buffered Saline tablets have been specifically developed for use in immunoassay procedures.


50, 100 tablets in poly bottle


One tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C.

General description

Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.

Price and Availability

Customers Also Viewed

BioPerformance Certified, pH 7.4

10× concentrate, BioPerformance Certified, suitable for cell culture

dry powder, pH 7.4, contains 3% nonfat milk

powder, pH 7.4

Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, High performance liquid chromatography, Ion Exchange, Microscopy, Precipitation, Purification, Scanning electron microscopy

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunohistochemistry, Immunoprecipitation, Molecular biology, Peptide synthesis, Phosphorylations, Purification, Western blot

Immunohistochemistry Procedures

Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues. In order to perform the standard staining procedure, first the tissue section has to be deparaffinized and ...
Keywords: Digestions, Immunohistochemistry, Solvents

Peer-Reviewed Papers


Set your institution to view full text papers.

Alzheimer's Aβ Peptides With Disease-associated N-terminal Modifications: Influence Of Isomerisation, Truncation And Mutation On Cu Coordination. Drew, S.C., et al. PLoS ONE 5, e15875, (2010)


SIRT5 regulation of ammonia-induced autophagy and mitophagy. Polletta L, Vernucci E, Carnevale I, et al. Autophagy 11(2), 253-70, (2015)


Multiplexed, label-free detection of biomarkers using aptamers and Tunable Resistive Pulse Sensing (AptaTRPS). Billinge ER, Platt M, et al. Biosens. Bioelectron. 68, 741-8, (2015)


Isolation and transplantation of sturgeon early-stage germ cells. Pšeni?ka M, Saito T, Linhartová Z, et al. Theriogenology 83(6), 1085-92, (2015)


A comparison of alternative assays to measure DNA damage in stallion spermatozoa: TUNEL test versus 'Nicoletti assay'. Kakasi B, Nagy S, Pál L, et al. Acta Vet. Hung. 63(1), 118-24, (2015)


Reduced axonal transport in Parkinson's disease cybrid neurites is restored by light therapy. Trimmer PA Molecular Neurodegeneration 4, 26, (2009)


Immunogenicity of human mesenchymal stem cells in HLA-class I-restricted T-cell responses against viral or tumor-associated antigens. Morandi F Stem Cells 26(5), 1275-87, (2008)


Nuclear entry of hyperbranched polylysine nanoparticles into cochlear cells. Weikai Zhang et al Int. J. Nanomedicine 6, 535-46, (2011)


Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis. Marlena Zielinska et al Int. J. Nanomedicine 6, 3163-72, (2011)


Toll-like receptor 4-dependent responses to lung injury in a murine model of pulmonary contusion. Hoth JJ Shock 31(4), 376-81, (2009)


Quantitative PCR for genetic markers of human fecal pollution. Shanks OC Appl. Environ. Microbiol. 75(17), 5507-13, (2009)


A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards. Sivaganesan M BMC Bioinformatics 9, 120, (2008)


Ionic components of electric current at rat corneal wounds. Vieira AC PLoS ONE 6(2), e17411, (2011)


Specificity of the horizontal cell-photoreceptor connections in the zebrafish (Danio rerio) retina. Yong N. Li et al J. Comp. Neurol. 516, 442-53, (2010)


Design and analysis of rhesus cytomegalovirus IL-10 mutants as a model for novel vaccines against human cytomegalovirus. Naomi J. Logsdon et al PLoS ONE 6, e28127, (2011)


Enhanced growth and osteogenic differentiation of human osteoblast-like cells on boron-doped nanocrystalline diamond thin films. Grausova L PLoS ONE 6(6), e20943, (2011)


The cytoplasmic domain of proEGF negatively regulates motility and elastinolytic activity in thyroid carcinoma cells. Glogowska A Neoplasia 10(10), 1120-30, (2008)


The role of H2O2 outer diffusion on the performance of implantable glucose sensors. Vaddiraju S Biosens. Bioelectron. 24(6), 1557-62, (2009)


WFDC1/ps20 is a novel innate immunomodulatory signature protein of human immunodeficiency virus (HIV)-permissive CD4+ CD45RO+ memory T cells that promotes infection by upregulating CD54 integrin expression and is elevated in HIV type 1 infection. R. Alvarez et al J. Virol. 82, 471-86, (2008)


Expression of the Pax2 transcription factor is associated with vestibular phenotype in the avian inner ear. Warchol ME Dev. Neurobiol. 69(2-3), 191-202, (2009)


Oral immunization with recombinant lactobacillus plantarum induces a protective immune response in mice with Lyme disease. del Rio B Clin. Vaccine Immunol. 15(9), 1429-35, (2008)


Automated quantification of DNA demethylation effects in cells via 3D mapping of nuclear signatures and population homogeneity assessment. Gertych A Cytometry. A 75(7), 569-83, (2009)


Reciprocal translocations in somatic and germ cells of mice chronically exposed by inhalation to ethylene oxide: implications for risk assessment. Donner EM Mutagenesis 25(1), 49-55, (2010)


The MP65 gene is required for cell wall integrity, adherence to epithelial cells and biofilm formation in Candida albicans. Sandini S BMC Microbiol. 11, 106, (2011)


Differential Expression of SPARC in Intestinal-type Gastric Cancer Correlates with Tumor Progression and Nodal Spread. Konrad Franke et al Transl. Oncol. 2, 310-20, (2009)


Insulin and IGF-I prevent brain atrophy and DNA loss in diabetes. Predrag Šerbedžija et al Brain Res. 1303, DOI: 10.1093/ecam/nen085, (2009)


Campylobacter jejuni in penguins, Antarctica. Griekspoor P Emerging Infect. Dis. 15(5), 847-8, (2009)


New method for the isolation of endothelial cells from large vessels. Ataollahi F, Pingguan-Murphy B, Moradi A, et al. Cytotherapy 16(8), 1145-52, (2014)


A self-avoidance mechanism in patterning of the urinary collecting duct tree. Davies JA, Hohenstein P, Chang CH, et al. BMC Dev. Biol. 14(1), 35, (2014)


Related Products


Product #


Add to Cart

CLS3516 Corning® Costar® cell culture plates, 6 well, flat bottom (Individually wrapped)
CLS2592 Corning® 1 x 8 Stripwell 96 well plates, high binding surface, clear
CLS430641 Corning® cell culture flasks, surface area 75 cm2, canted neck, cap (vented)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?