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P4417 Sigma

Phosphate buffered saline


Synonym: PBS



Frequently Asked Questions

Frequently Asked Questions are available for this Product.


Phosphate Buffered Saline tablets have been specifically developed for use in immunoassay procedures.


50, 100 tablets in poly bottle


One tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C.

General description

Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.

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Flow Cytometry Instruments

All labs need water

SNAP and Go!
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Immunohistochemistry Procedures

Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues. In order to perform the standard staining procedure, first the tissue section has to be deparaffinized and ...
Keywords: Digestions, Immunohistochemistry, Solvents

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