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P4417 Sigma

Phosphate buffered saline

tablet

Synonym: PBS

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Description

Frequently Asked Questions

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Application

Phosphate Buffered Saline tablets have been specifically developed for use in immunoassay procedures.

Reconstitution

One tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C.

General description

Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.

Price and Availability

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TWEEN<SUP>®</SUP> 20

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Safety & Documentation

Safety Information

WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, High performance liquid chromatography, Ion Exchange, Microscopy, Precipitation, Purification, Scanning electron microscopy

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
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Peer-Reviewed Papers

References

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Alzheimer's Aβ Peptides With Disease-associated N-terminal Modifications: Influence Of Isomerisation, Truncation And Mutation On Cu Coordination. Drew, S.C., et al. PLoS ONE 5, e15875, (2010)

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Reduced axonal transport in Parkinson's disease cybrid neurites is restored by light therapy. Trimmer PA Molecular Neurodegeneration 4, 26, (2009)

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Immunogenicity of human mesenchymal stem cells in HLA-class I-restricted T-cell responses against viral or tumor-associated antigens. Morandi F Stem Cells 26(5), 1275-87, (2008)

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Nuclear entry of hyperbranched polylysine nanoparticles into cochlear cells. Weikai Zhang et al Int. J. Nanomedicine 6, 535-46, (2011)

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Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis. Marlena Zielinska et al Int. J. Nanomedicine 6, 3163-72, (2011)

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Toll-like receptor 4-dependent responses to lung injury in a murine model of pulmonary contusion. Hoth JJ Shock 31(4), 376-81, (2009)

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Quantitative PCR for genetic markers of human fecal pollution. Shanks OC Appl. Environ. Microbiol. 75(17), 5507-13, (2009)

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A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards. Sivaganesan M BMC Bioinformatics 9, 120, (2008)

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Ionic components of electric current at rat corneal wounds. Vieira AC PLoS ONE 6(2), e17411, (2011)

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Specificity of the horizontal cell-photoreceptor connections in the zebrafish (Danio rerio) retina. Yong N. Li et al J. Comp. Neurol. 516, 442-53, (2010)

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New method for the isolation of endothelial cells from large vessels. Ataollahi F, Pingguan-Murphy B, Moradi A, et al. Cytotherapy 16(8), 1145-52, (2014)

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Design and analysis of rhesus cytomegalovirus IL-10 mutants as a model for novel vaccines against human cytomegalovirus. Naomi J. Logsdon et al PLoS ONE 6, e28127, (2011)

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Enhanced growth and osteogenic differentiation of human osteoblast-like cells on boron-doped nanocrystalline diamond thin films. Grausova L PLoS ONE 6(6), e20943, (2011)

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The cytoplasmic domain of proEGF negatively regulates motility and elastinolytic activity in thyroid carcinoma cells. Glogowska A Neoplasia 10(10), 1120-30, (2008)

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The role of H2O2 outer diffusion on the performance of implantable glucose sensors. Vaddiraju S Biosens. Bioelectron. 24(6), 1557-62, (2009)

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WFDC1/ps20 is a novel innate immunomodulatory signature protein of human immunodeficiency virus (HIV)-permissive CD4+ CD45RO+ memory T cells that promotes infection by upregulating CD54 integrin expression and is elevated in HIV type 1 infection. R. Alvarez et al J. Virol. 82, 471-86, (2008)

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Expression of the Pax2 transcription factor is associated with vestibular phenotype in the avian inner ear. Warchol ME Dev. Neurobiol. 69(2-3), 191-202, (2009)

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Oral immunization with recombinant lactobacillus plantarum induces a protective immune response in mice with Lyme disease. del Rio B Clin. Vaccine Immunol. 15(9), 1429-35, (2008)

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Automated quantification of DNA demethylation effects in cells via 3D mapping of nuclear signatures and population homogeneity assessment. Gertych A Cytometry. A 75(7), 569-83, (2009)

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Reciprocal translocations in somatic and germ cells of mice chronically exposed by inhalation to ethylene oxide: implications for risk assessment. Donner EM Mutagenesis 25(1), 49-55, (2010)

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The MP65 gene is required for cell wall integrity, adherence to epithelial cells and biofilm formation in Candida albicans. Sandini S BMC Microbiol. 11, 106, (2011)

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Differential Expression of SPARC in Intestinal-type Gastric Cancer Correlates with Tumor Progression and Nodal Spread. Konrad Franke et al Transl. Oncol. 2, 310-20, (2009)

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A self-avoidance mechanism in patterning of the urinary collecting duct tree. Davies JA, Hohenstein P, Chang CH, et al. BMC Dev. Biol. 14(1), 35, (2014)

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Insulin and IGF-I prevent brain atrophy and DNA loss in diabetes. Predrag Šerbedžija et al Brain Res. 1303, DOI: 10.1093/ecam/nen085, (2009)

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Campylobacter jejuni in penguins, Antarctica. Griekspoor P Emerging Infect. Dis. 15(5), 847-8, (2009)

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