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P4439 Sigma

Phosphatase, Alkaline from porcine kidney

lyophilized powder, ≥100 DEA units/mg protein

Synonym: Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Alkaline Phosphatase, Application Index, Biochemicals and Reagents,
form   lyophilized powder
composition   Protein, ≥50%
storage temp.   −20°C

Description

Analysis Note

Protein determined by biuret.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.

Packaging

100 units in serum bottle

Package size based on DEA units

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

Physical form

Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

To standardize a procedure for the enzymatic assay of alkaline phosphatase, diethanolamine assay.
Keywords: Enzyme activity, Extinction coefficient, PAGE

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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substrate

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N2770 SIGMAFAST p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL
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