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P4458 Sigma

Penicillin-Streptomycin

Solution Stabilized, with 5,000 units penicillin and 5mg streptomycin/mL, sterile-filtered, BioReagent, suitable for cell culture

Synonym: Pen-Strep

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Properties

Related Categories Antibacterial, Antibiotic Solutions, Antibiotics, Antibiotics A to Z, Antibiotics N-S,
InChI Key   KMOOCZWLFBSQCW-XFOLRDBHSA-N
sterility   sterile-filtered
product line   BioReagent
form   liquid
concentration   50 ×
impurities   endotoxin, tested
suitability   suitable for cell culture
Mode of action   cell wall synthesis | interferes
  protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

This product is formulated to contain 5,000 units penicillin and 5 mg streptomycin/mL solubilized in a proprietary citrate buffer.

Biochem/physiol Actions

Mode of Action: Penicillin-Streptomycin acts in two ways. Penicillin inhibits bacterial cell wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.

Application

Penicillin-Streptomycin is used to supplement cell culture media to control bacterial contamination. It is recommended for use in cell culture applications at 10 mL/L.

Other Notes

20ml,100ml

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the method of preparation for Product No. P4458 (Penicillin-Streptomycin); what solution is it made in?
Product No. P4458 is not simply penicillin and streptomycin dissolved in water; it is made in a proprietary buffer.
What is the concentration of the P4458 Penicillin-Streptomycin solution? For example, is it a 50X or 100X?
Product No. P4458 is designed to be added directly to media; you should add 20mL to 1L of medium.  There is no intermediate dilution step. So the concentration 50X refers to the 50X dilution into media to the final working concentration.
What is the storage temperature and the stability of the P4458 Penicillin-Streptomycin?
Product No. P4458 (Penicillin-Streptomycin solution) should be stored in the freezer at -20 °C for long-term storage. It may be kept refrigerated at 2-8 °C for up to 40 days.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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