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P4475 Sigma

ReadyMix Taq PCR Reaction Mix

without MgCl2



Related Categories Molecular Biology, PCR/Amplification, Routine PCR Amplification More...
concentration   1.5 units/reaction (50 μL reaction volume)
shipped in   wet ice
storage temp.   −20°C


Features and Benefits

• Amplifies targets up to 7 kb in length

• ReadyMix Taq PCR Reaction Mix offers the same great performance as Taq DNA Polymerase in a more convenient formulation

• ReadyMix format reduces setup time.

• Reduced risk of contamination from multiple pipet steps.

General description

ReadyMix Taq PCR Reaction Mix is a prepared solution containing everything needed for a PCR reaction except the specific primers and template. The mix includes Sigma′s high quality Taq DNA Polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. For reaction set-up, add the ReadyMix (25 μL) to the primers, template and water (total volume 50 μL). Using ReadyMix Taq PCR Reaction Mix reduces pipetting steps and risk of contamination. This saves time and reduces errors while still providing the great performance of Sigma′s Taq DNA Polymerase.


Supplied with a vial of PCR grade water for dilution.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Legal Information

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Kapa Biosystems

All labs need water
Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Protocols & Articles

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Peer-Reviewed Papers


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