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P4475 Sigma

ReadyMix Taq PCR Reaction Mix

without MgCl2

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Properties

Related Categories Molecular Biology, PCR/Amplification, Routine PCR Amplification More...
concentration   1.5 units/reaction (50 μL reaction volume)
shipped in   wet ice
storage temp.   −20°C

Description

Features and Benefits

• Amplifies targets up to 7 kb in length

• ReadyMix Taq PCR Reaction Mix offers the same great performance as Taq DNA Polymerase in a more convenient formulation

• ReadyMix format reduces setup time.

• Reduced risk of contamination from multiple pipet steps.

General description

ReadyMix Taq PCR Reaction Mix is a prepared solution containing everything needed for a PCR reaction except the specific primers and template. The mix includes Sigma′s high quality Taq DNA Polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. For reaction set-up, add the ReadyMix (25 μL) to the primers, template and water (total volume 50 μL). Using ReadyMix Taq PCR Reaction Mix reduces pipetting steps and risk of contamination. This saves time and reduces errors while still providing the great performance of Sigma′s Taq DNA Polymerase.

Packaging

Supplied with a vial of PCR grade water for dilution.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Legal Information

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Kapa Biosystems
Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Related Content

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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