P4557 Sigma

Phenol solution

Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology



Related Categories Acids and Bases, Biochemicals and Reagents, Denaturation, Molecular Biology, Plant Biotechnology,
grade   for molecular biology
product line   BioReagent
form   liquid
storage temp.   2-8°C
Gene Information   human ... GABRA1(2554)


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


• 1 bottle Tris-equilabrated Phenol (product number P4557)
• Equilibration Buffer (product B5658)

General description

A tris-equilabrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications, including nucleic acid extractions using standard phenol-chloroform protocols.


100, 400 mL in glass bottle


Phenol solution is suitable for use in nucleic acid extraction using standard phenol-choloroform protocols.

Price and Availability

Safety & Documentation

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option:
What is the molarity of Phenol solution, Product P4557?
The Molarity is ~10.6.  The density of the phenol is 1.05g/ml, and the molecular weight is 94.11.
I see that there are two layers in the bottle Phenol solution, Product P4557. Which layer do I use?
The top phase is Tris buffer, so use the lower/bottom phase.
What do I do to equilibrate the Phenol solution, Product P4557, to around pH 8?
This phenol solution (Product No. P4557) includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. After addition of the buffer to the phenol, it may take about 6 hours (mixture can be kept at 2-8 °C) until the bottom phase (phenol) becomes clear. Some of the haziness in the phenol phase may be due to some air bubbles caused by mixing by inversion. There could be a hazy interface between the phenol and aqueous phase which remains after the separation.  The entire contents of the bottle of the buffer should be added to the phenol. If buffering less than the complete bottle, use at a ratio of 6.5 mL buffer to 100 mL phenol. For 10 mL of phenol, use 650 μL of buffer. For 1 mL of phenol, use 65 μL of buffer.
What is the final pH of Phenol solution, Product P4557, after adding the buffer?
The pH is typically 8.0; the specifications state a range of 7.7-8.1.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles


Phenol solution (equilibrated with 10mM Tris HCl, pH 8.0, with 1mM EDTA)

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. 

Peer-Reviewed Papers


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