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PCR Applications: Protocols for Functional Genomics

  •  ISBN-10 0-12-372186-5

  •  ISBN-13 978-0-12-372186-0

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Related Categories Books and Software, Labware, Molecular Biology Books, PCR Books
publication info   M.A. Innis, et al., ed., Academic Press, 1999, 584 pp., hard cover
mfr. no.   Elsevier

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General description

From ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences, PCR Applications examines the latest developments in this field. It includes statistical refinement of primer design parameters, techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology.

Table of Contents

Key Concepts for PCR:
Optimization of PCR: Conversations between Michael and David.
The Convergence of PCR,
Computers, and the Human Genome Project: Past, Present and Future.
Thermostable DNA Polymerases: An Update.
Musings on Microbial Genomes. Statistical Refinement of Primer Design Parameters.
Multiplex PCR: Optimization Guidelines.
The Use of Immobilized Mismatch Binding Protein for the Optimization of PCR Fidelity.
A New Generation of PCR Instruments and Nucleic Acid Concentration Systems.
Sequencing PCR Products. Recent Advances in High-Temperature Reverse Transcription and PCR.
Viral Genotyping by a Quantitative Point Mutation Assay: Application to HIV-1 Drug Resistance.
In Situ PCR. Quantitative PCR:
Standards for PCR Assays. Rapid Thermal Cycling and PCR Kinetics.
Kinetics of Competitive Reverse Transcriptase-PCR.
Kinetic PCR Analysis Using a CCD Camera and without Using Oligonucleotide Probes.
Quantification of Telomerase Activity Using Telomeric Repeat Amplification Protocol.
Gene Discovery: Differential Display. Single-Cell cDNA Libraries. Whole Cell Assays.
Screening Differentially Displayed PCR Products by Single-Strand Conformation Polymorphism Gels.
Microsatellite Protocols.
Real-Time Quantitative PCR: Uses in Discovery Research.
Homology Cloning: A Molecular Taxonomy of the Archaea.
Cloning Mammalian Homologs of Drosophila Genes.
Cloning Human Homologs of Yeast Genes.
Genomics and Expression Profiling: Cellular Transcriptome Analysis Using a Kinetic PCR Assay.
Parallel Analysis with Biological Chips.
High Density cDNA Grids for Hybridization Fingerprinting Experiments.
Comparative Genomic Hybridization.
Genetic Footprinting and Functional Maps of the Yeast Genome.
Molecular Analysis of Microdissected Tissue: Laser Capture Microdissection.
A Fluorescent, Multiplex Solid-Phase MiniSequencing Method for Genotyping Cytochrome P450 Genes.
The Cleavase Enzyme for Mutation and Polymorphism Scanning.
Index.

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