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P4630 Sigma

Protease from bovine pancreas

Type I, ≥5 units/mg solid

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Proteases, Proteases & Protein Sequencing More...
type   Type I
storage temp.   −20°C

Description

Packaging

1, 5, 10 g in poly bottle

250 mg in poly bottle

Quality

Crude

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Application

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Product P4630 is from bovine pancreas. Protease from bovine pancrease (type I) has been used for the extraction of hemicellulose.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

Protocols & Applications

Non-specific Protease Activity Assay Video

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
UK9540000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Enzymatic Assay of Protease Using Casein As a Substrate

To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate at Sigma-Aldrich St. Louis.

Peer-Reviewed Papers
15

References

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Description

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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