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P4656 Sigma

Pepsinogen from porcine stomach

Grade I-S, lyophilized powder, ~3,000 units/mg protein (after activation to pepsin at pH 2.0 at 25°C)

Synonym: Pepsinogen from hog stomach

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Proteases, Proteases & Protein Sequencing More...
type   Grade I-S
form   lyophilized powder
purified by   chromatography
foreign activity   pepsin, free
storage temp.   2-8°C

Description

Analysis Note

Protein determined by biuret.

Unit Definition

One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products, using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)

Biochem/physiol Actions

Pepsinogen is the zymogen of pepsin. It is processed by autocatalytic cleavage of 44 amino acids to generate active pepsin. Serum levels of pepsinogen have been measured to identify gastric cancer risk.

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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