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P4691 Sigma

Protein G-Agarose, Fast Flow from Streptococcus sp.

saline suspension



Related Categories Affinity Chromatography, Affinity Resins, Antibodies, Antibody Purification, Antibody Purification and Characterization,
form   saline suspension
extent of labeling   ≥2 mg per mL
matrix   Highly cross-linked 4% beaded agarose, fast flow
matrix activation   cyanogen bromide
matrix attachment   amino
matrix spacer   1 atom
capacity   ≥20 mg/mL binding capacity (human IgG (I 4506))(in 20 mM sodium phosphate, pH 7.0)
storage temp.   2-8°C


Preparation Note

Prepared with a genetically engineered Protein G which retains its high affinity for IgG and lacks albumin and Fab binding sites and membrane binding regions.

Physical form

Suspension in 0.5 M NaCl containing 20% ethanol as a preservative


Protein G-agarose is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, protein A, G and L resins, and purification and detection. Protein G-agarose has been used to study breast cancer and falsely elevated thyroid-stimulating hormone (TSH).

Price and Availability

Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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