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P4707 Sigma

Poly-L-lysine solution

mol wt 70,000-150,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

Synonym: PDL Solution

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Properties

Related Categories Amino Acids, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture,
Quality Level   PREMIUM
sterility   sterile-filtered
product line   BioReagent
mol wt   mol wt 70,000-150,000
concentration   0.01%
impurities   endotoxin, tested
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Application

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

• Poly-L-lysine coated glass coverslips have been used for mounting samples for scanning electron microscopy.
• Poly-L-lysine coated wells were used for trapping of PC-12 cells on electrodes.
• Poly-L-lysine-coated borosilicate glass slides were used for the seeding of viral vector-transduced cells.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Preparation Note

This product is supplied as a 0.01% sterile filtered solution in water, using a molecular weight of 70 - 150 kDa. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the concentration of Product P4707, Poly-L-Lysine solution?
This product is supplied as a 0.01% w/v solution.
What molecular weight Poly-L-Lysine product should I choose?
The lower molecular weight poly-L-lysine (30-70 kDa) is easier to use because it is less viscous in solution, but the higher molecular weight poly-L-lysine (>300 kDa) provides more attachment sites per molecule. The mid-range product has a molecular weight range of 70-150 kDa.
Should I use Poly-L-Lysine or Poly-D-Lysine?
Certain cells secrete proteases, which can digest poly-L-lysine. For those cell types, poly-D-lysine should be used.
What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat slides?
For coating slides with poly-L-lysine, in general, it is recommended to use a 0.01% (w/v) solution. After a 5 minute incubation, the excess solution is removed and the slides are dried at room temperature or in an oven at gentle heat.  More information on this procedure can be found on the Product Information Sheet for P4707: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/1/p4707pis..pdf
What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat tissue culture plasticware?
A solution of 0.1 mg/mL is prepared; 0.5 mL of that solution is aseptically transferred to a non-tissue culture treated 25 square cm flask. The flask is gently rocked to evenly coat the surface. After five minutes, the excess solution is removed and the surface is thoroughly rinsed with sterile water and allowed to dry for several hours.  More information can be found on the Product Information Sheet for P4707: http://www.sigmaaldrich.com/sigma/product%20information%20sheet/P4707pis.pdf
How can I sterilize slides coated with Product P4707, Poly-L-Lysine solution?
Poly-L-Lysine coated slides can be exposed to UV light overnight for sterilization.
How should I dilute Product P4707, Poly-L-Lysine solution?
For coating slides with poly-L-lysine, in general, it is recommended to use a 0.01% (w/v) solution.  However, if your protocol requires a more dilute solution, you can add sterile water or sterile buffer like Phosphate Buffered Saline.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
Show more questions
Protocols & Articles

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Poly-L-Lysine Cell Attachment Protocol

From our library of Articles, Sigma-Aldrich presents Poly-L-Lysine Cell Attachment Protocol
George Sitterley
BioFiles 2008, 3.8, 12.

Poly-lysine

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Peer-Reviewed Papers
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References

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