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P4707 Sigma

Poly-L-lysine solution

mol wt 70,000-150,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

Synonym: PDL Solution

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Properties

Related Categories Amino Acids, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture,
Quality Level   PREMIUM
sterility   sterile-filtered
product line   BioReagent
mol wt   mol wt 70,000-150,000
concentration   0.01%
impurities   endotoxin, tested
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Application

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Preparation Note

This product is supplied as a 0.01% sterile filtered solution in water, using a molecular weight of 70 - 150 kDa. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

Price and Availability


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All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Poly-L-Lysine Cell Attachment Protocol

From our library of Articles, Sigma-Aldrich presents Poly-L-Lysine Cell Attachment Protocol
George Sitterley
BioFiles 2008, 3.8, 12.

Poly-lysine

Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are depend...
George Sitterley
BioFiles 2008, 3.8, 12.
Keywords: Adhesion, Cell culture, Enzyme-linked immunosorbent assay

The Cancer Stem Cell Hypothesis

Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
Keywords: Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Cell signaling, Clinical, Degradations, Environmental, Gastrointestinal, Gene expression, Ligands, Phosphorylations, Poisons, Transcription, Transfection, transformation

Related Content

癌症干细胞

传统观点认为,癌症是环境或内部因素诱导正常细胞内的关键致癌基因和肿瘤抑制基因突变导致的一种疾病。当这些突变导致细胞向更原始、高度增殖状态转化,克隆扩张形成白血病或实体肿瘤时,就会出现癌症的临床表现1。然而,这种模式不能够完全解释许多肿瘤和转移瘤的长发育延迟,产生细胞去分化和细胞永生的起始诱导机制,或肿瘤本身内的细胞功能和表型多样性起源。过去十年中,越来越多的证据表明,恶性肿瘤起源于组织干细胞在突...
Keywords: Angiogenesis, Cancer, Eliminations, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations

Peer-Reviewed Papers
15

References

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