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P4742 Sigma

Protein Protocols Handbook, 2nd ed.

  •  ISBN-10 0-89603-941-2

  •  ISBN-13 978-0-89603-941-4

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publication info   J.M. Walker, Humana Press, 2002, 850 pp., soft cover
mfr. no.   Humana

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General description

This edition provides a cross-section of analytical techniques commonly used for proteins and peptides. The text is significantly updated and expands on a number of chapters from the first edition. The Notes section complements the Methods by indicating how best to deal with any problem or difficulty that may arise when using a given technique. Further chapters examine the developments in the use of mass spectrometry in protein characterization. Recognition of the central role of 2-D PAGE in proteomics has resulted in an expansion of chapters on this subject, as well as a number of new techniques for staining and analyzing protein blots.

Table of Contents

PARTIAL Part I. Quantitation of Proteins
1 Protein Determination by UV Absorption
2 The Lowry Method for Protein Quantitation
3 The Bicinchoninic Acid (BCA) Assay for Protein Quantitation
4 The Bradford Method for Protein Quantitation
5 Ultrafast Protein Determinations Using Microwave Enhancement
6 The Nitric Acid Method for Protein Estimation in Biological Samples
7 Quantitation of Tryptophan in Proteins
8 Flow Cytometric Quantitation of Cellular Proteins
9 Kinetic Silver Staining of Proteins Part II. Electrophoresis of Proteins and Peptides and Detection in Gels
10 Nondenaturing Polyacrylamide Gel Electrophoresis of Proteis
11 SDS Polyacrylamide Gel Electrophoresis of Proteins
12 Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins
13 SDS-Polyacrylamide Gel Electrophoresis of Peptides
14 Identification of Nucleic Acid Binding Proteins Using Nondenaturing Sodium Decyl Sulfate Polyacrylamide Gel Electrophoresis (SDecS-Page)
15 Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins: Mr-Based Separation of Proteins with Retained Native Activity
16 Acetic–Acid–Urea Polyacrylamide Gel Electrophoresis of Basic Proteins
17 Acid–Urea–Triton Polyacrylamide Gel Electrophoresis of Histones
18 Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels
19 Protein Solubility in Two-Dimensional Electrophoresis: Basic Principles and Issues
20 Preparation of Protein Samples from Mouse and Human Tissues for 2-D Electrophoresis
21 Radiolabeling of Eukaryotic Cells and Subsequent Preparation for 2-D Electrophoresis
22 Two-Dimensional Polyacrylamide Gel Electrophoresis Using Carrier Ampholyte pH Gradients in the First Dimension
23 Casting Immobilized pH Gradients (IPGs)
24 Nonequilibrium pH Gel Electrophoresis (NEPHGE)
25 Difference Gel Electrophoresis
26 Comparing 2-D Electrophoretic Gels Across Internet Databases
27 Immunoblotting of 2-D Electrophoresis Separated Proteins
28 Quantification of Radiolabeled Proteins in Polyacrylamide Gels
29 Quantification of Proteins on Polyacrylamide Gels
30 Rapid and Sensitive Staining of Unfixed Proteins in Polyacrylamide Gels with Nile Red
31 Zinc-Reverse Staining Technique
32 Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels
33 Detection of Proteins in Polyacrylamide Gels by Silver Staining
34 Protein Detection on Polyacrylamide Gels and Electroblots Using Transition Metal Chelate Stains
35 Detection of Proteins in Polyacrylamide Gels by Fluorescent Staining
36 Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using Eosin Y Stain
37 Electroelution of Proteins from Polyacrylamide Gels
38 Autoradiography and Fluorography of Acrylamide Gels
Part III. Blotting and Detection Methods
39 Protein Blotting by Electroblotting
40 Protein Blotting by Semidry Method
41 Protein Blotting by the Capillary Method 42 43 44 45 46 47 48 49 50 51 52 53. 54 55 56 57 58
Part IV. Chemical Modification of Proteins and Peptide 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74. 75 76 77 78
Part V. Protein/Peptide 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103
Part VI. Glycoproteins 104 105 106 107 108 109 110 111 112 113 114 115 116 117118 119 120 121 122 123 124 125 126 127
Part. VII. Antibody Techniques 128 129 130 131 132 133. 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157
Part VIII. Monoclonal Antibodies 158. 159 160 161 162 163 164.

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