Synonym: 4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate
|Related Categories||p-Nitrophenyl Phosphate Substrate Systems, Alkaline Phosphatase, Alphabetical Listing, Biochemicals and Reagents, Colorimetric,|
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Substrate for the determination of acid and alkaline phosphatases.
1, 5, 10, 25, 50 g in glass bottle
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powder, BioReagent, suitable for cell culture, ≥97%
ready to use solution
5 mg tablets
Certificate of Analysis
Certificate of Origin
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
1. OBJECTIVE To standardize a procedure for the enzymatic assay of alkaline phosphatase, diethanolamine assay.
Keywords: Enzyme activity, Extinction coefficient, PAGE
Fine antigenic specificity and cooperative bactericidal activity of monoclonal antibodies directed at the meningococcal vaccine candidate factor h-binding protein. Beernink PT Infect. Immun. 76(9), 4232-40, (2008)
Export of functional Streptomyces coelicolor alditol oxidase to the periplasm or cell surface of Escherichia coli and its application in whole-cell biocatalysis. van Bloois E Appl. Microbiol. Biotechnol. 83(4), 679-87, (2009)
J.P. Bretaudiere and T. Spillman, H.U. Bergmeyer, ed. Methods of Enzymatic Analysis 3rd ed., Weinheim 4, (1984)
O.H. Lowry Meth. Enzymol. 4, (1957)
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