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P4789 Sigma

Protease from Bacillus sp.

Type XXVII, lyophilized powder, 7-14 units/mg solid

Synonym: Nagarse

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Properties

type   Type XXVII
form   lyophilized powder
purified by   crystallization
foreign activity   DNase, RNase, essentially free
storage temp.   −20°C

Description

Analysis Note

Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin BPN′.

Biochem/physiol Actions

Bacterial protease

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
UK9540000
Protocols & Articles

Protocols

Enzymatic Assay of Protease Using Casein As a Substrate

To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate at Sigma-Aldrich St. Louis.

Peer-Reviewed Papers
15

References

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P5380 Protease from Bacillus licheniformis, Type VIII, lyophilized powder, 7-15 units/mg solid

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