Poly-L -lysine solution
mol wt 150,000-300,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture
DOWNLOAD MSDS (PDF)Synonym: PDL Solution
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CAS Number 25988-63-0
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MDL number MFCD00165424
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Popular Documents: RAMAN FTIR (PDF)
Properties
| Related Categories | Amino Acids, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture, |
| Quality Level | GMP |
| sterility | sterile-filtered |
| product line | BioReagent |
| mol wt | mol wt 150,000-300,000 |
| concentration | 0.01% |
| impurities | endotoxin, tested |
| suitability | suitable for cell culture |
Description
Application
Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.
Other Notes
This poly-L-lysine is relatively high density which provides good binding characteristics, but it is more viscous and difficult to handle. It may be degraded by some cell types. In that case a poly-D-lysine should be chosen.
For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.
General description
Poly-lysine polymers are useful molecules to prepare surfaces for cell attachment. Poly-lysine polymers are available in L and D conformations. Poly-L-lysine polymers can be digested by some cell types, whereas poly-D-lysines are stable. Poly-lysine polymers from 70 to 300 kDa provide sufficient attachment site density for good cell adherence. However, higher molecular weight poly-lysine polymers are more viscous and difficult to work with.
The choice of poly-lysine polymer for a specific cell type and application should account for the susceptibility of the polymer to digestion, the required attachment site density and the difficulty in handling.
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