|Related Categories||Amino Acids, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture,|
|mol wt||mol wt 150,000-300,000|
|suitability||suitable for cell culture|
Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.
This poly-L-lysine is relatively high density which provides good binding characteristics, but it is more viscous and difficult to handle. It may be degraded by some cell types. In that case a poly-D-lysine should be chosen.
For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.
Poly-lysine polymers are useful molecules to prepare surfaces for cell attachment. Poly-lysine polymers are available in L and D conformations. Poly-L-lysine polymers can be digested by some cell types, whereas poly-D-lysines are stable. Poly-lysine polymers from 70 to 300 kDa provide sufficient attachment site density for good cell adherence. However, higher molecular weight poly-lysine polymers are more viscous and difficult to work with.
The choice of poly-lysine polymer for a specific cell type and application should account for the susceptibility of the polymer to digestion, the required attachment site density and the difficulty in handling.
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From our library of Articles, Sigma-Aldrich presents Poly-L-Lysine Cell Attachment Protocol
BioFiles 2008, 3.8, 12.
Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are depend...
BioFiles 2008, 3.8, 12.
Keywords: Adhesion, Cell culture, Enzyme-linked immunosorbent assay
Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
BioFiles 2008, 3.5, 4.
Keywords: Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Clinical, DNA replication, Degradations, Eliminations, Environmental, Gastrointestinal, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations, Poisons, Transcription, Transfection, transformation
Establishment of B-cell lines latently infected with reactivation-competent murine gammaherpesvirus 68 provides evidence for viral alteration of a DNA damage-signaling cascade. Forrest JC and Speck SH J. Virol. 82(15), 7688-99, (2008)
Soluble factors from neocortical astrocytes enhance neuronal differentiation of neural progenitor cells from adult rat hippocampus on micropatterned polymer substrates. Oh J J. Biomed. Mater. Res. A 91(2), 575-85, (2009)
Growth inhibitory and anti-tumour activities of OSU-03012, a novel PDK-1 inhibitor, on vestibular schwannoma and malignant schwannoma cells. Lee TX, Packer MD, Huang J, et al. Eur. J. Cancer 45(9), 1709-20, (2009)
Functional diversity of human basic helix-loop-helix transcription factor TCF4 isoforms generated by alternative 5' exon usage and splicing. Sepp M, Kannike K, Eesmaa A, et al. PLoS ONE 6(7), e22138, (2011)
Lack of a-disintegrin-and-metalloproteinase ADAM10 leads to intracellular accumulation and loss of shedding of the cellular prion protein in vivo. Altmeppen HC, Prox J, Puig B, et al. Molecular Neurodegeneration 6, 36, (2011)
β-hydroxybutyrate abrogates formation of bovine neutrophil extracellular traps and bactericidal activity against mammary pathogenic Escherichia coli. Navit Grinberg et al Infect. Immun. 76, 2802-7, (2008)
Candidate polyanionic microbicides inhibit human T-cell lymphotropic virus type 1 receptor interactions, cell-free infection, and cell-cell spread. Daniela Romer et al Antimicrob. Agents Chemother. 53, 678-87, (2009)
Suspension fluorescence in situ hybridization (S-FISH) combined with automatic detection and laser microdissection for STR profiling of male cells in male/female mixtures. Mado Vandewoestyne et al Int. J. Legal Med. 123, 441-7, (2009)
NRF2 activation restores disease related metabolic deficiencies in olfactory neurosphere-derived cells from patients with sporadic Parkinson's disease. Anthony L. Cook et al PLoS ONE 6, e21907, (2011)
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