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P4860 Sigma

Protease from Bacillus licheniformis

≥2.4 U/g

Synonym: Alcalase® 2.4L, Proteinase from Bacillus licheniformis, Subtilisin A

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

Activity expressed in Anson Units

Legal Information

A product of Novozyme Corp.

Alcalase is a registered trademark of Novozymes Corp.

Application

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations.

The enzyme from Sigma has been used to prepare chicken (leg) bone protein hydrolysates while evaluating ACE inhibitory peptides. It has also been used for the hydrolysis of Chlorella pyrenoidosa protein extracts during a study of their antitumor activities.

Biochem/physiol Actions

Alcalase is an endo-protease of the serine type. It has a broad substrate specificity and can hydrolyze most peptide bonds within a protein molecule. It is active between pH 6.5 and 8.5 and has an optimum temperature of 60 °C. The enzyme is is used in detergent formulations to remove protein-based stains.

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

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Safety & Documentation

Safety Information

Signal word 
Danger
Hazard statements 
Personal Protective Equipment 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
1
RTECS 
CO9550000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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