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P4890 Sigma

Protein Kinase, 3′,5′-cyclic AMP dependent from rabbit muscle

lyophilized powder, ~0.2 unit/μg protein (activity is reduced at least 5-fold in the absence of cAMP)

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Properties

form   lyophilized powder
composition   Protein, ~98%
storage temp.   −20°C

Description

Analysis Note

Protein determined by biuret.

Packaging

Package size based on protein content

Preparation Note

Prepared essentially by procedure of Beavo, J.A., et al., Methods in Enzymology, Vol. XXXVIII, 301: Section A [through 2nd DEAE cellulose column] (1974). Academic Press, N.Y. This is predominantly the "Peak I protein kinase."

Unit Definition

One unit will transfer 1.0 picomole phosphate from γ-32P-ATP to hydrolyzed and partially dephosphorylated casein, per min at pH 6.5 at 30°C in the presence of 0.006 mM cyclic AMP.

Physical form

Crude lyophilized powder containing 2% potassium phosphate, pH 7.0

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
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