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P4932 Sigma

Polymyxin B sulfate salt

powder, BioReagent, suitable for cell culture

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Properties

Related Categories Antibacterial, Antibiotic powders, Antibiotics, Antibiotics A to Z, Antibiotics N-S,
product line   BioReagent
form   powder
potency   ≥6,000 USP units per mg
solubility   H2O: soluble50 mg/mL
suitability   suitable for cell culture
antibiotic activity spectrum   Gram-negative bacteria
storage temp.   2-8°C

Description

Application

Polymyxin B sulfate is a strongly cationic cyclic polypeptide antibiotic that is derived from fermentation of Bacilus polymyxa. It is a mixture of B1 and B2 sulfate. The product has been used clinically to treat infections of the urinary tract, meninges and blood stream caused by susceptible strains of Pseudomonas aeruginosa. It also has uses studying multidrug-resistant pathogens, as an immobilized agent for removal of endotoxins, and to induce pore formation in the membranes of cortex cells from excised sorghum roots.

Biochem/physiol Actions

Mode of Action: Polymyxin B Sulfate binds to the lipid A portion of bacterial lipopolysaccharides†, disrupting the cytoplasmic membrane by inducing pores large enough to permit nucleotide leakage in bacterial walls. This disrupts the permeability of the cytoplasmic membrane.

Antimicrobial spectrum: Has bactericidal action on most gram-negative bacilli††, including E. Coli and on most fungi and gram-positive bacteria.

Caution

As supplied, the product should be stored at 2-8°C in the dark. No change was observed in retained samples after three years′ of storage in these conditions. Stock solutions should be sterile filtered and stored at 2-8°C. They are stable at 37°C for 5 days.

Preparation Note

Polymyxin B sulfate is soluble in water at 50 mg/mL, producing a very slightly hazy, colorless to yellow solution. It has only minimal solubility in any organic solvent. For example, it has a solubility of 0.115 mg/mL in ethanol.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

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Biotechnology Performance Certified

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
Hazard Codes (Europe) 
Xn
Risk Statements (Europe) 
22
WGK Germany 
3
RTECS 
TR1150000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Antibiotic Selection Guide

1Application Key: CC = Callus Culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant selection; SE = somatic embryogenesis; SSC = stem selection culture;...
Keywords: Antibiotics, Cell culture

Peer-Reviewed Papers

References

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1. Effects of sulfomethylation on the mechanism of action of colimycin towards "Escherichia coli" B (author's transl). Cornu, J. Ann. Microbiol. 131B, 121, (1980)

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2. Polymyxin and related peptide antibiotics. D.R. Storm et al. Annu. Rev. Biochem. 46, 723, (1977)

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3. Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. D.C. Morrison and D.M. Jacobs Immunochemistry 13, 813, (1976)

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4. Lerner, H.R., et al. Physiol. Plant. 57, 90, (1983)

Characterization of the polymyxin B resistome of Pseudomonas aeruginosa. Fernández L, Alvarez-Ortega C, Wiegand I, et al. Antimicrob. Agents Chemother. 57(1), 110-9, (2013)

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Postoperative polymyxin B hemoperfusion and mortality in patients with abdominal septic shock: a propensity-matched analysis. Iwagami M, Yasunaga H, Doi K, et al. Crit. Care Med. 42(5), 1187-93, (2014)

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Pharmacokinetics of polymyxin B in patients on continuous venovenous haemodialysis. Sandri AM, Landersdorfer CB, Jacob J, et al. J. Antimicrob. Chemother. 68(3), 674-7, (2013)

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Gut microbiota. Antimicrobial peptide resistance mediates resilience of prominent gut commensals during inflammation. Cullen TW, Schofield WB, Barry NA, et al. Science 347(6218), 170-5, (2015)

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Impact of prothrombin time-International Normalized Ratio on outcome of patients with septic shock receiving polymyxin B cartridge hemoperfusion. Ishizuka M, Tago K, and Kubota K Surgery 156(1), 168-75, (2014)

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[Case report; A case of acute respiratory failure following influenza virus infection successfully treated by direct hemoperfusion using a polymyxin B immobilized fiber column]. Arai D, Fukunaga K, Fujii K, et al. Nippon. Naika Gakkai Zasshi. 102(2), 429-32, (2013)

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Vascular malformation of the glans penis successfully treated with Nd:YAG laser. López V, Ricart JM, López I, et al. Dermatol. Online J. 18(10), 16, (2012)

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Conjugated polymer-coated bacteria for multimodal intracellular and extracellular anticancer activity. Zhu C, Yang Q, Lv F, et al. Adv. Mater. 25(8), 1203-8, (2013)

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Elucidating the regulon of multidrug resistance regulator RarA in Klebsiella pneumoniae. De Majumdar S, Veleba M, Finn S, et al. Antimicrob. Agents Chemother. 57(4), 1603-9, (2013)

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Optimizing hollow-fiber-based pharmacokinetic assay via chemical stability study to account for inaccurate simulated drug clearance of rifampicin. New LS, Lim TP, Oh JW, et al. Anal. Bioanal. Chem 405(4), 1407-15, (2013)

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Colistin: an update on the antibiotic of the 21st century. Biswas S, Brunel JM, Dubus JC, et al. Expert Rev. Anti. Infect. Ther. 10(8), 917-34, (2012)

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Polymyxin B sulfate and colistin: old antibiotics for emerging multiresistant Gram-negative bacteria. Evans, M.E., et al. Ann. Pharmacother. 33, 960-967, (1999)

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The major allergen of the Parietaria pollen contains an LPS-binding region with immuno-modulatory activity. Bonura A, Corinti S, Schiavi E, et al. Allergy 68(3), 297-303, (2013)

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10'(Z),13'(E)-heptadecadienylhydroquinone inhibits swarming and virulence factors and increases polymyxin B susceptibility in Proteus mirabilis. Liu MC, Lin SB, Chien HF, et al. PLoS ONE 7(9), e45563, (2012)

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Endotoxin adsorption by polymyxin B column or intraaortic balloon pumping use for severe septic cardiomyopathy. Nakamura K, Doi K, Inokuchi R, et al. Am. J. Emerg. Med. 31(5), 893.e1-3, (2013)

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Development of a selective enrichment broth supplemented with bacteriological charcoal and a high concentration of polymyxin B for the detection of Campylobacter jejuni and Campylobacter coli in chicken carcass rinses. Chon JW, Kim H, Yim JH, et al. Int. J. Food Microbiol. 162(3), 308-10, (2013)

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Successful treatment of severe amiodarone pulmonary toxicity with polymyxin B-immobilized fiber column direct hemoperfusion. Sato N, Kojima K, Horio Y, et al. Chest 143(4), 1146-50, (2013)

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Cost-effectiveness of perioperative selective decontamination of the digestive tract versus placebo in elective gastrointestinal surgery. Dijksman LM, Roos D, Gerhards MF, et al. Dig. Surg. 29(5), 384-90, (2012)

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Acute otitis externa: an update. Schaefer P and Baugh RF Am. Fam. Physician 86(11), 1055-61, (2012)

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Type 6 secretion system-mediated immunity to type 4 secretion system-mediated gene transfer. Ho BT, Basler M, and Mekalanos JJ Science 342(6155), 250-3, (2013)

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Successful polymyxin B hemoperfusion treatment associated with serial reduction of serum anti-CADM-140/MDA5 antibody levels in rapidly progressive interstitial lung disease with amyopathic dermatomyositis. Teruya A, Kawamura K, Ichikado K, et al. Chest 144(6), 1934-6, (2013)

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Nephrotoxicity and efficacy assessment of polymyxin use in 92 transplant patients. Mostardeiro MM, Pereira CA, Marra AR, et al. Antimicrob. Agents Chemother. 57(3), 1442-6, (2013)

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Use of a surfactant (polysorbate 80) to improve MIC susceptibility testing results for polymyxin B and colistin. Sader HS, Rhomberg PR, Flamm RK, et al. Diagn. Microbiol. Infect. Dis. 74(4), 412-4, (2012)

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An allele of an ancestral transcription factor dependent on a horizontally acquired gene product. Chen HD, Jewett MW, and Groisman EA PLoS Genet. 8(12), e1003060, (2012)

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An improved selective culture medium enhances the isolation of Burkholderia pseudomallei from contaminated specimens. Goodyear A, Strange L, Rholl DA, et al. Am. J. Trop. Med. Hyg. 89(5), 973-82, (2013)

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Biophysical characterization of polymyxin B interaction with LPS aggregates and membrane model systems. Domingues MM, Inácio RG, Raimundo JM, et al. Biopolymers 98(4), 338-44, (2012)

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Evaluation of the anticryptococcal activity of the antibiotic polymyxin B in vitro and in vivo. Zhai B and Lin X Int. J. Antimicrob. Agents 41(3), 250-4, (2013)

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Continuous hemodiafiltration with a cytokine-adsorbing hemofilter for sepsis. Hirasawa H, Oda S, Nakamura M, et al. Blood Purif. 34(2), 164-70, (2012)

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Intraventricular polymyxin B for the treatment of neonatal meningo-ventriculitis caused by multi-resistant Acinetobacter baumannii--case report and review of literature. Piparsania S, Rajput N, and Bhatambare G Turk. J. Pediatr. 54(5), 548-54, (2012)

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A single-blinded randomized clinical trial comparing polymyxin B-trimethoprim and moxifloxacin for treatment of acute conjunctivitis in children. Williams L, Malhotra Y, Murante B, et al. J. Pediatr. 162(4), 857-61, (2013)

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Streptococcus pyogenes polymyxin B-resistant mutants display enhanced ExPortal integrity. Port GC, Vega LA, Nylander AB, et al. J. Bacteriol. 196(14), 2563-77, (2014)

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The interaction of polysaccharide from Auricularia polytricha with quantum dots and the protection of plasmid DNA from damage. Wang W, Zhang G, and Zou J Appl. Biochem. Biotechnol. 169(8), 2263-72, (2013)

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Clearance of nasal Staphylococcus aureus colonization with triple antibiotic ointment. Grossman EM, Nanda S, Gordon JR, et al. Journal. of. Drugs in. Dermatology. 11(12), 1490-2, (2012)

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Direct hemoperfusion with a polymyxin B column versus vasopressin for gram negative septic shock: a matched cohort study of the effect on survival. Sawa N, Ubara Y, Sumida K, et al. Clin. Nephrol. 79(6), 463-70, (2013)

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Polymyxin B hemoperfusion: matching the cure to the disease. Cruz DN Crit. Care Med. 42(5), 1309-10, (2014)

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The novel polymyxin derivative NAB739 is remarkably less cytotoxic than polymyxin B and colistin to human kidney proximal tubular cells. Vaara M and Vaara T Int. J. Antimicrob. Agents 41(3), 292-3, (2013)

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Merck 14,7573

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