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P4932 Sigma

Polymyxin B sulfate salt

powder, BioReagent, suitable for cell culture

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Properties

Related Categories Antibacterial, Antibiotic powders, Antibiotics, Antibiotics A to Z, Antibiotics N-S,
product line   BioReagent
form   powder
potency   ≥6,000 USP units per mg
solubility   H2O: soluble50 mg/mL
suitability   suitable for cell culture
Mode of action   cell membrane | interferes
antibiotic activity spectrum   Gram-negative bacteria
storage temp.   2-8°C

Description

Biochem/physiol Actions

Mode of Action: Polymyxin B Sulfate binds to the lipid A portion of bacterial lipopolysaccharides†, disrupting the cytoplasmic membrane by inducing pores large enough to permit nucleotide leakage in bacterial walls. This disrupts the permeability of the cytoplasmic membrane.

Antimicrobial spectrum: Has bactericidal action on most gram-negative bacilli††, including E. Coli and on most fungi and gram-positive bacteria.

Caution

As supplied, the product should be stored at 2-8°C in the dark. No change was observed in retained samples after three years′ of storage in these conditions. Stock solutions should be sterile filtered and stored at 2-8°C. They are stable at 37°C for 5 days.

Preparation Note

Polymyxin B sulfate is soluble in water at 50 mg/mL, producing a very slightly hazy, colorless to yellow solution. It has only minimal solubility in any organic solvent. For example, it has a solubility of 0.115 mg/mL in ethanol.

Application

Polymyxin B sulfate salt has been used -
• for the treatment of BAC-1.2F5 murine macrophages as an LPS (lipopolysaccharide) inhibitor
• as positive and negative control during microbicidal concentration (MCC) assay

General description

Polymyxin B is a member of the polymyxin group of antibiotics, obtained from different strains of Bacillus polymyxa and related species. It contains a cyclic heptapeptide unit and a tripeptide side chain, which is N-acylated by a fatty acyl residue. Polymyxin B sulfate salt is generally used for the treatment of Gram-negative bacterial infections.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability


Polymeric Drug Delivery Techniques Guide

Filter out uncertainty.

All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
TR1150000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

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Antibiotic Selection Guide

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Keywords: Antibiotics, Cell culture

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