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P4957 Sigma

Poly-L-ornithine solution

mol wt 30,000-70,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

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Properties

Related Categories Amino Acids, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture,
Quality Level   PREMIUM
sterility   sterile-filtered
product line   BioReagent
mol wt   mol wt 30,000-70,000
concentration   0.01%
impurities   endotoxin, tested
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Other Notes

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Preparation Note

Prepared in cell culture grade water.

Application

Poly-L-ornithine has been used successfully for the attachement and study of neural cell differentiation and outgrowth. Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Neural Stem Cell FAQs

The use of neural stem cells (NSCs) in biomedical research is becoming increasingly popular, resulting in breakthrough studies rejected the longstanding belief that neuronal tissue is incapable of re...
Keywords: Cell culture, Central Nervous System, Centrifugation, Clinical, Diffusion, Digestions, Diseases, Gene expression, Immunocytochemistry, Neurodegenerative Diseases, Parkinson Disease

Poly-lysine

Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are depend...
George Sitterley
BioFiles 2008, 3.8, 12.
Keywords: Adhesion, Cell culture, Enzyme-linked immunosorbent assay

The Cancer Stem Cell Hypothesis

Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
Keywords: Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Cell signaling, Clinical, Degradations, Environmental, Gastrointestinal, Gene expression, Ligands, Phosphorylations, Poisons, Transcription, Transfection, transformation

Protocols

3D Neural Stem Cell Models of Alzheimer’s Disease Protocol

Introduction Protocol    • Human ReNcell® VM NSC Maintenance and Passaging    • Viral Infection of Human ReNcell® VM NSCs    • Enrichment of High-Expressing Transgenic Human ReNcell® VM NSCs    • Cul...
Keywords: AGE, Alzheimer Disease, Apoptosis, Cell culture, Cell proliferation, Centrifugation, Deposition, Drug discovery, Flow cytometry, Gene expression, Immunohistochemistry, Microscopy, Optical microscopy, Transcription, Western blot

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传统观点认为,癌症是环境或内部因素诱导正常细胞内的关键致癌基因和肿瘤抑制基因突变导致的一种疾病。当这些突变导致细胞向更原始、高度增殖状态转化,克隆扩张形成白血病或实体肿瘤时,就会出现癌症的临床表现1。然而,这种模式不能够完全解释许多肿瘤和转移瘤的长发育延迟,产生细胞去分化和细胞永生的起始诱导机制,或肿瘤本身内的细胞功能和表型多样性起源。过去十年中,越来越多的证据表明,恶性肿瘤起源于组织干细胞在突...
Keywords: Angiogenesis, Cancer, Eliminations, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations

Peer-Reviewed Papers
15

References

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