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P4957 Sigma

Poly-L-ornithine solution

mol wt 30,000-70,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

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Properties

Related Categories Amino Acids, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture,
Quality Level   PREMIUM
sterility   sterile-filtered
product line   BioReagent
mol wt   mol wt 30,000-70,000
concentration   0.01%
impurities   endotoxin, tested
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Other Notes

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Preparation Note

Prepared in cell culture grade water.

Application

Poly-L-ornithine has been used successfully for the attachement and study of neural cell differentiation and outgrowth. Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

3D-Cell culture: An overview of advanced tools and techniques

Cell culture techniques are ubiquitous in areas of developmental biology, drug discovery, regenerative medicine and protein production. Since the introduction of cell culture techniques, cells have b...
Keywords: Alkylations, Apoptosis, Biomaterials, Cell culture, Cell proliferation, Clinical, Cytotoxins, Drug discovery, Gene expression, Metabolism, Pharmaceutical

Cancer Stem Cells: New Targets for Cancer Therapy

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

Neural Stem Cell FAQs

The use of neural stem cells (NSCs) in biomedical research is becoming increasingly popular, resulting in breakthrough studies rejected the longstanding belief that neuronal tissue is incapable of re...
Keywords: Cell culture, Central Nervous System, Centrifugation, Clinical, Diffusion, Digestions, Diseases, Gene expression, Immunocytochemistry, Neurodegenerative Diseases, Parkinson Disease

Poly-lysine

Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are depend...
George Sitterley
BioFiles 2008, 3.8, 12.
Keywords: Adhesion, Cell culture, Enzyme-linked immunosorbent assay

Protocols

3D Neural Stem Cell Models of Alzheimer’s Disease Protocol

Introduction Protocol    • Human ReNcell® VM NSC Maintenance and Passaging    • Viral Infection of Human ReNcell® VM NSCs    • Enrichment of High-Expressing Transgenic Human ReNcell® VM NSCs    • Cul...
Keywords: AGE, Alzheimer Disease, Apoptosis, Cell culture, Cell proliferation, Centrifugation, Deposition, Drug discovery, Flow cytometry, Gene expression, Immunohistochemistry, Microscopy, Optical microscopy, Transcription, Western blot

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Peer-Reviewed Papers
15

References

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