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P4978 Sigma

Phosphatase, Alkaline from calf intestine

buffered aqueous glycerol solution

Synonym: CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Alkaline Phosphatase Enzymes, Analytical and Industrial Enzymes, Biochemicals and Reagents,
grade   for molecular biology
form   buffered aqueous glycerol solution
mol wt   mol wt ~80 kDa
concentration   ≥10,000 units/mL
foreign activity   DNase, RNase, none detected
shipped in   wet ice
storage temp.   −20°C
Gene Information   cow ... ALPI(280993)

Description

Components

Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.

Application

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

General description

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Other Notes

Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.

Unit Definition

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Alkaline Phosphatase, Calf Intestinal (CIP)

Product Description Product Summary Storage buffer Procedure: Dephosphorylation of DNA Heat Inactivation Materials Reference
Keywords: Cloning, Peptide synthesis, Precipitation, Purification

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

To standardize a procedure for the enzymatic assay of alkaline phosphatase, diethanolamine assay.
Keywords: Enzyme activity, Extinction coefficient, PAGE

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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substrate

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N1891 SIGMAFAST p-Nitrophenyl phosphate Tablets, tablet, To prepare 5 mL
M3168 4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System, liquid

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