Phosphatase, Alkaline from calf intestine
buffered aqueous glycerol solution
DOWNLOAD MSDS (PDF)Synonym:
CIAP,
Orthophosphoric-
-
CAS Number 9001-78-9
-
MDL number MFCD00131849
-
Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
| Properties
| Related Categories | 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Alkaline Phosphatase Enzymes, Analytical and Industrial Enzymes, Biochemicals and Reagents, |
| form | buffered aqueous glycerol solution |
| concentration | ≥10,000 units/mL in diethanolamine buffer, pH 9.8 at 37°C |
| foreign activity | DNase, RNase, none detected |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | cow ... ALPI(280993) |
Description
Biochem/physiol Actions
Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.
Caution
Alkaline phosphatase in 5 mM EDTA, pH 8, will be irreversibly heat inactivated at 75 °C for 10 minutes.
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.
Physical form
Solution in 10 mM Tris-HCl, pH 8.2, 50 mM KCl, 1 mM MgCl2, 0.1 mM ZnCl2, 50% (w/v) glycerol.
Application
Calf intestinal alkaline phosphatase is used in molecular biology applications to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations.
Price and Availability
Customers Also Viewed
lyophilized powder, ≥200 units/mg protein (E
BioUltra, buffered aqueous glycerol solution, ≥6,500 DEA units/mg protein
ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Bulk Ordering & Pricing:
Need larger quantities for your development, manufacturing or research applications?
