|Related Categories||3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Alkaline Phosphatase Enzymes, Analytical and Industrial Enzymes, Biochemicals and Reagents,|
|grade||for molecular biology|
|form||buffered aqueous glycerol solution|
|mol wt||mol wt ~80 kDa|
|concentration||≥10,000 units/mL in diethanolamine buffer, pH 9.8 at 37°C|
|foreign activity||DNase, RNase, none detected|
|shipped in||wet ice|
|Gene Information||cow ... ALPI(280993)|
Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2) 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol.
Commonly used to remove the 5′-terminal phosphate from nucleic acids.
Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.
Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.
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lyophilized powder, ≥200 units/mg protein (E
BioUltra, buffered aqueous glycerol solution, ≥6,500 DEA units/mg protein
ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
1. OBJECTIVE To standardize a procedure for the enzymatic assay of alkaline phosphatase, diethanolamine assay.
Keywords: Enzyme activity, Extinction coefficient, PAGE
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
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Bone marrow concentrated cell transplantation: rationale for its use in the treatment of human osteochondral lesions. Cavallo C, Desando G, Cattini L, et al. J. Biol. Regul. Homeost. Agents 27(1), 165-75, (2013)
Proliferation and osteo/odontoblastic differentiation of stem cells from dental apical papilla in mineralization-inducing medium containing additional KH(2)PO(4). Wang L, Yan M, Wang Y, et al. Cell Prolif. 46(2), 214-22, (2013)
Hypomorphic mutations in PGAP2, encoding a GPI-anchor-remodeling protein, cause autosomal-recessive intellectual disability. Hansen L, Tawamie H, Murakami Y, et al. Am. J. Hum. Genet. 92(4), 575-83, (2013)
Effect of incorporation of nanoscale bioactive glass and hydroxyapatite in PCL/chitosan nanofibers for bone and periodontal tissue engineering. Shalumon KT, Sowmya S, Sathish D, et al. J. Biomed. Nanotechnol. 9(3), 430-40, (2013)
Livolin ameliorates elevations in alanine transaminase in HIV infected patients commencing highly active antiretroviral therapy. Otegbayo JA, Kuti MA, Ogunbode O, et al. Afr. J. Med. Med. Sci. 41(4), 417-22, (2012)
Thiazolidinediones cause compaction of nuclear heterochromatin in the pluripotent mesenchymal cell line C3H10T1/2 when inducing an adipogenic phenotype. Chappard D, Marchand-Libouban H, Moreau MF, et al. Anal. Quant. Cytol. Histol. 35(2), 85-94, (2013)
Studies on calf-intestinal alkaline phosphatase. I. Chromatographic purification, microheterogeneity and some other properties of the purified enzyme. ENGSTROM L Biochim. Biophys. Acta 52, 36-48, (1961)
In vitro ALP and osteocalcin gene expression analysis and in vivo biocompatibility of N-methylene phosphonic chitosan nanofibers for bone regeneration. Datta P, Ghosh P, Ghosh K, et al. J. Biomed. Nanotechnol. 9(5), 870-9, (2013)
Design and characterization of a recombinant colorimetric SAG1-alkaline phosphatase conjugate to detect specific antibody responses against Toxoplasma gondii. Chahed Bel-Ochi N, Bouratbine A, and Mousli M J. Immunol. Methods 394(1-2), 107-14, (2013)
Notch1 promotes the pro-osteogenic response of human aortic valve interstitial cells via modulation of ERK1/2 and nuclear factor-κB activation. Zeng Q, Song R, Ao L, et al. Arterioscler. Thromb. Vasc. Biol. 33(7), 1580-90, (2013)
Effect of strontium ranelate on serum leptin and bone turnover markers in women with established postmenopausal osteoporosis. Bayhan I, Dogan NU, Ozaksit G, et al. J. Reprod. Med. 58(7-8), 319-23, (2013)
Protective effect of Spirulina and tamarind fruit pulp diet supplement in fish (Gambusia affinis Baird & Girard) exposed to sublethal concentration of fluoride, aluminum and aluminum fluoride. Sharma KP, Upreti N, Sharma S, et al. Indian J. Exp. Biol. 50(12), 897-903, (2012)
Biochemical markers of bone turnover and clinical outcome in patients with renal cell and bladder carcinoma with bone metastases following treatment with zoledronic acid: The TUGAMO study. Alcaraz A, González-López R, Morote J, et al. Br. J. Cancer 109(1), 121-30, (2013)
Reid, T.W., et al. Enzymes 3, 373-416, (1971)
Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY , (1989), 5.72
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|D6518||Diethylenetriaminepentaacetic acid, ≥99% (titration)|
|P9375||1,10-Phenanthroline monohydrate, reagent grade|
|ED2SS||Ethylenediaminetetraacetic acid disodium salt dihydrate, reagent grade, 98.5-101.5% (titration)|
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|P6911||Protease from Streptomyces griseus, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)|
|LIG1||DNA Ligation Kit|
|D9380||DNA Polymerase I from Escherichia coli lysogenic for NM 964, buffered aqueous glycerol solution|
|R0884||T7 RNA Polymerase, recombinant, expressed in E. coli, buffered aqueous solution|
|D8276||DNA Polymerase I, Klenow Fragment from Escherichia coli, buffered aqueous glycerol solution|
|D2886||DNA Ligase from T4-infected Escherichia coli, buffered aqueous glycerol solution|
|T4427||Terminal Transferase from calf thymus, buffered aqueous glycerol solution|
|P4390||Polynucleotide Kinase from T4-infected Escherichia coli, 10 units/μL, buffered aqueous glycerol solution|
|E1131||Exonuclease III from Escherichia coli BE25 /psGR3, buffered aqueous glycerol solution|
|G5663||Glutathione S-Transferase from Schistosoma japonicum, recombinant, expressed in E. coli, buffered aqueous solution|
|D7291||Deoxyribonuclease I RNase-free solution from bovine pancreas|
|T7428||Topoisomerase I from wheat germ, buffered aqueous glycerol solution|
|R6513||Ribonuclease A from bovine pancreas, salt-free, lyophilized powder|
|R4642||Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)|
|R0759||RNA Polymerase from Salmonella enterica serotype typhimurium, SP6-infected, buffered aqueous solution|
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