• USA Home
  • P5306 - Papain from papaya latex

EMAIL THIS PAGE TO A FRIEND
P5306 Sigma

Papain from papaya latex

lyophilized powder, aseptically filled

Synonym: Papainase

Purchase

Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
sterility   aseptically filled
form   lyophilized powder
activity   ≥8.0 units/mg protein
mol wt   mol wt 21 kDa
solubility   H2O: soluble1.2 mg/mL
storage temp.   −20°C

Description

Application

Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies . It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P5306 is provided as a lyophilized powder that is aseptically filled. It has been used to digest mouse retinas to study the RAGE axis in nonproliferative diabetic retinopathy (NPDR).

The product from Sigma has been used in the digestion of extracellular matrix (ECM). It has also been used with other proteolytic enzymes for in situ protein degradation to produce crystals suitable for structure determination.

Biochem/physiol Actions

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Papain papaya latex has antifungal activity against C. albicans. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

Other Notes

View more information on papain at www.sigma-aldrich.com/enzymeexplorer.

Preparation Note

Prepared from P 4762

Price and Availability


Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
RU4950000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

inhibitor

Product #

Description

Add to Cart

E3132 E-64, protease inhibitor
M6159 α2-Macroglobulin from human plasma, BioUltra, ≥98% (SDS-PAGE)
D7910 3,4-Dichloroisocoumarin, serine protease inhibitor
E3520 Ebselen, cysteine modifier
L2023 Leupeptin trifluoroacetate salt, ≥90% (HPLC), microbial
A6191 Antipain dihydrochloride from microbial source, protease inhibitor
C8917 Cystatin from chicken egg white, lyophilized powder
G5386 Gly-Gly-Tyr-Arg, ≥97% (HPLC)
C7268 Chymostatin, microbial

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

substrate

Product #

Description

Add to Cart

P3169 pGlu-Phe-Leu p-nitroanilide, protease substrate

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?